BCL9L Dysfunction Impairs Caspase-2 Expression Permitting Aneuploidy Tolerance in Colorectal Cancer

Carlos López-García; Laurent Sansregret; Enric Domingo; Nicholas McGranahan; Sebastijan Hobor; Nicolai Juul Birkbak; Stuart Horswell; Eva Grönroos; Francesco Favero; Andrew J. Rowan; Nicholas Matthews; Sharmin Begum; Benjamin Phillimore; Rebecca Burrell; Dahmane Oukrif; Bradley Spencer-Dene; Michal Kovac; Gordon Stamp; Aengus Stewart; Havard Danielsen; Marco Novelli; Ian Tomlinson; Charles Swanton

doi:10.1016/j.ccell.2016.11.001

BCL9L Dysfunction Impairs Caspase-2 Expression Permitting Aneuploidy Tolerance in Colorectal Cancer

Carlos López-García, Laurent Sansregret, Enric Domingo, Nicholas McGranahan, Sebastijan Hobor, Nicolai Juul Birkbak, Stuart Horswell, Eva Grönroos, Francesco Favero, Andrew J. Rowan, Nicholas Matthews, Sharmin Begum, Benjamin Phillimore, Rebecca Burrell, Dahmane Oukrif, Bradley Spencer-Dene, Michal Kovac, Gordon Stamp, Aengus Stewart, Havard DanielsenMarco Novelli, Ian Tomlinson, Charles Swanton

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

Chromosomal instability (CIN) contributes to cancer evolution, intratumor heterogeneity, and drug resistance. CIN is driven by chromosome segregation errors and a tolerance phenotype that permits the propagation of aneuploid genomes. Through genomic analysis of colorectal cancers and cell lines, we find frequent loss of heterozygosity and mutations in BCL9L in aneuploid tumors. BCL9L deficiency promoted tolerance of chromosome missegregation events, propagation of aneuploidy, and genetic heterogeneity in xenograft models likely through modulation of Wnt signaling. We find that BCL9L dysfunction contributes to aneuploidy tolerance in both TP53-WT and mutant cells by reducing basal caspase-2 levels and preventing cleavage of MDM2 and BID. Efforts to exploit aneuploidy tolerance mechanisms and the BCL9L/caspase-2/BID axis may limit cancer diversity and evolution.

Original language	English
Journal	Cancer Cell
Volume	31
Issue number	1
Pages (from-to)	79-93
Number of pages	15
ISSN	1535-6108
DOIs	https://doi.org/10.1016/j.ccell.2016.11.001
Publication status	Published - 2016

Keywords

aneuploidy tolerance
chromosomal instability
BCL9L
caspase-2
p53
intratumor heterogeneity
chromosome segregation errors
mitotic checkpoint
BID
colorectal cancer evolution

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10.1016/j.ccell.2016.11.001

BCL9LFinal published version, 2.49 MBLicence: CC BY

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López-García, C., Sansregret, L., Domingo, E., McGranahan, N., Hobor, S., Birkbak, N. J., Horswell, S., Grönroos, E., Favero, F., Rowan, A. J., Matthews, N., Begum, S., Phillimore, B., Burrell, R., Oukrif, D., Spencer-Dene, B., Kovac, M., Stamp, G., Stewart, A., ... Swanton, C. (2016). BCL9L Dysfunction Impairs Caspase-2 Expression Permitting Aneuploidy Tolerance in Colorectal Cancer. Cancer Cell, 31(1), 79-93. https://doi.org/10.1016/j.ccell.2016.11.001

López-García, Carlos ; Sansregret, Laurent ; Domingo, Enric ; McGranahan, Nicholas ; Hobor, Sebastijan ; Birkbak, Nicolai Juul ; Horswell, Stuart ; Grönroos, Eva ; Favero, Francesco ; Rowan, Andrew J. ; Matthews, Nicholas ; Begum, Sharmin ; Phillimore, Benjamin ; Burrell, Rebecca ; Oukrif, Dahmane ; Spencer-Dene, Bradley ; Kovac, Michal ; Stamp, Gordon ; Stewart, Aengus ; Danielsen, Havard ; Novelli, Marco ; Tomlinson, Ian ; Swanton, Charles. / BCL9L Dysfunction Impairs Caspase-2 Expression Permitting Aneuploidy Tolerance in Colorectal Cancer. In: Cancer Cell. 2016 ; Vol. 31, No. 1. pp. 79-93.

@article{7eacfaeb7942428c93e72e8f9fc64914,

title = "BCL9L Dysfunction Impairs Caspase-2 Expression Permitting Aneuploidy Tolerance in Colorectal Cancer",

abstract = "Chromosomal instability (CIN) contributes to cancer evolution, intratumor heterogeneity, and drug resistance. CIN is driven by chromosome segregation errors and a tolerance phenotype that permits the propagation of aneuploid genomes. Through genomic analysis of colorectal cancers and cell lines, we find frequent loss of heterozygosity and mutations in BCL9L in aneuploid tumors. BCL9L deficiency promoted tolerance of chromosome missegregation events, propagation of aneuploidy, and genetic heterogeneity in xenograft models likely through modulation of Wnt signaling. We find that BCL9L dysfunction contributes to aneuploidy tolerance in both TP53-WT and mutant cells by reducing basal caspase-2 levels and preventing cleavage of MDM2 and BID. Efforts to exploit aneuploidy tolerance mechanisms and the BCL9L/caspase-2/BID axis may limit cancer diversity and evolution.",

keywords = "aneuploidy tolerance, chromosomal instability, BCL9L, caspase-2, p53, intratumor heterogeneity, chromosome segregation errors, mitotic checkpoint, BID, colorectal cancer evolution",

author = "Carlos L{\'o}pez-Garc{\'i}a and Laurent Sansregret and Enric Domingo and Nicholas McGranahan and Sebastijan Hobor and Birkbak, {Nicolai Juul} and Stuart Horswell and Eva Gr{\"o}nroos and Francesco Favero and Rowan, {Andrew J.} and Nicholas Matthews and Sharmin Begum and Benjamin Phillimore and Rebecca Burrell and Dahmane Oukrif and Bradley Spencer-Dene and Michal Kovac and Gordon Stamp and Aengus Stewart and Havard Danielsen and Marco Novelli and Ian Tomlinson and Charles Swanton",

year = "2016",

doi = "10.1016/j.ccell.2016.11.001",

language = "English",

volume = "31",

pages = "79--93",

journal = "Cancer Cell",

issn = "1535-6108",

publisher = "Cell Press",

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López-García, C, Sansregret, L, Domingo, E, McGranahan, N, Hobor, S, Birkbak, NJ, Horswell, S, Grönroos, E, Favero, F, Rowan, AJ, Matthews, N, Begum, S, Phillimore, B, Burrell, R, Oukrif, D, Spencer-Dene, B, Kovac, M, Stamp, G, Stewart, A, Danielsen, H, Novelli, M, Tomlinson, I & Swanton, C 2016, 'BCL9L Dysfunction Impairs Caspase-2 Expression Permitting Aneuploidy Tolerance in Colorectal Cancer', Cancer Cell, vol. 31, no. 1, pp. 79-93. https://doi.org/10.1016/j.ccell.2016.11.001

BCL9L Dysfunction Impairs Caspase-2 Expression Permitting Aneuploidy Tolerance in Colorectal Cancer. / López-García, Carlos; Sansregret, Laurent; Domingo, Enric; McGranahan, Nicholas; Hobor, Sebastijan; Birkbak, Nicolai Juul; Horswell, Stuart; Grönroos, Eva; Favero, Francesco; Rowan, Andrew J.; Matthews, Nicholas; Begum, Sharmin; Phillimore, Benjamin; Burrell, Rebecca; Oukrif, Dahmane; Spencer-Dene, Bradley; Kovac, Michal; Stamp, Gordon; Stewart, Aengus; Danielsen, Havard; Novelli, Marco; Tomlinson, Ian; Swanton, Charles.

In: Cancer Cell, Vol. 31, No. 1, 2016, p. 79-93.

Research output: Contribution to journal › Journal article › Research › peer-review

TY - JOUR

T1 - BCL9L Dysfunction Impairs Caspase-2 Expression Permitting Aneuploidy Tolerance in Colorectal Cancer

AU - López-García, Carlos

AU - Sansregret, Laurent

AU - Domingo, Enric

AU - McGranahan, Nicholas

AU - Hobor, Sebastijan

AU - Birkbak, Nicolai Juul

AU - Horswell, Stuart

AU - Grönroos, Eva

AU - Favero, Francesco

AU - Rowan, Andrew J.

AU - Matthews, Nicholas

AU - Begum, Sharmin

AU - Phillimore, Benjamin

AU - Burrell, Rebecca

AU - Oukrif, Dahmane

AU - Spencer-Dene, Bradley

AU - Kovac, Michal

AU - Stamp, Gordon

AU - Stewart, Aengus

AU - Danielsen, Havard

AU - Novelli, Marco

AU - Tomlinson, Ian

AU - Swanton, Charles

PY - 2016

Y1 - 2016

N2 - Chromosomal instability (CIN) contributes to cancer evolution, intratumor heterogeneity, and drug resistance. CIN is driven by chromosome segregation errors and a tolerance phenotype that permits the propagation of aneuploid genomes. Through genomic analysis of colorectal cancers and cell lines, we find frequent loss of heterozygosity and mutations in BCL9L in aneuploid tumors. BCL9L deficiency promoted tolerance of chromosome missegregation events, propagation of aneuploidy, and genetic heterogeneity in xenograft models likely through modulation of Wnt signaling. We find that BCL9L dysfunction contributes to aneuploidy tolerance in both TP53-WT and mutant cells by reducing basal caspase-2 levels and preventing cleavage of MDM2 and BID. Efforts to exploit aneuploidy tolerance mechanisms and the BCL9L/caspase-2/BID axis may limit cancer diversity and evolution.

AB - Chromosomal instability (CIN) contributes to cancer evolution, intratumor heterogeneity, and drug resistance. CIN is driven by chromosome segregation errors and a tolerance phenotype that permits the propagation of aneuploid genomes. Through genomic analysis of colorectal cancers and cell lines, we find frequent loss of heterozygosity and mutations in BCL9L in aneuploid tumors. BCL9L deficiency promoted tolerance of chromosome missegregation events, propagation of aneuploidy, and genetic heterogeneity in xenograft models likely through modulation of Wnt signaling. We find that BCL9L dysfunction contributes to aneuploidy tolerance in both TP53-WT and mutant cells by reducing basal caspase-2 levels and preventing cleavage of MDM2 and BID. Efforts to exploit aneuploidy tolerance mechanisms and the BCL9L/caspase-2/BID axis may limit cancer diversity and evolution.

KW - aneuploidy tolerance

KW - chromosomal instability

KW - BCL9L

KW - caspase-2

KW - p53

KW - intratumor heterogeneity

KW - chromosome segregation errors

KW - mitotic checkpoint

KW - BID

KW - colorectal cancer evolution

U2 - 10.1016/j.ccell.2016.11.001

DO - 10.1016/j.ccell.2016.11.001

M3 - Journal article

C2 - 28073006

VL - 31

SP - 79

EP - 93

JO - Cancer Cell

JF - Cancer Cell

SN - 1535-6108

IS - 1

ER -