Batch variation between branchial cell cultures: An analysis of variance

Heinz Johs. Max Hansen, M. Grosell, L. Kristensen

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    Abstract

    We present in detail how a statistical analysis of variance (ANOVA) is used to sort out the effect of an unexpected batch-to-batch variation between cell cultures. Two separate cultures of rainbow trout branchial cells were grown on permeable filter supports (“inserts”). They were supposed to be simple duplicates for testing the effect of two induced factors—apical or basolateral addition of radioactive precursors and different apical media–on the incorporation of 14C-acetate and 32P-phosphate into tissue lipids. Unfortunately, they did not altogether give the same result. By accepting this fact and introducing the difference between batches as one of the factors in an expanded three-dimensional ANOVA, we were able to overcome an otherwise crucial lack of sufficiently reproducible duplicate values. We could thereby show that the effect of changing the apical medium was much more marked when the radioactive lipid precursors were added on the apical, rather than on the basolateral, side. The insert cell cultures were obviously polarized. We argue that it is not reasonable to reject troublesome experimental results, when we do not know a priori that something went wrong. The ANOVA is a very useful statistical tool that can be utilized by any experimentalist who cares to make the effort.
    Original languageEnglish
    Place of PublicationRoskilde
    PublisherRisø National Laboratory
    Number of pages20
    ISBN (Print)87-550-3161-7
    ISBN (Electronic)87-550-3161-7
    Publication statusPublished - 2003
    SeriesDenmark. Forskningscenter Risoe. Risoe-R
    Number1384(EN)
    ISSN0106-2840

    Keywords

    • Risø-R-1384
    • Risø-R-1384(EN)

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