Batch fermentations on synthetic mixed sugar and starch medium with amylolytic lactic acid bacteria

Mette Hedegaard Thomsen, J.P. Guyot, P. Kiel

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    The green crop drying industry in Denmark uses Italian rye grass, clover, and alfalfa as raw materials for the production of green pellets. The green crop drying industry solves its energy economical problems by heating and pressing of the green crop before drying. The produced sidestream is called brown juice. Brown juice was shown to be an excellent medium for lactic acid fermentation. The aim of this study was to investigate the utilisation of brown juice in the production of polylactic acid, where wheat starch would be added to increase the lactic acid yield and, thus, the feasibility of the process. A number of amylolytic lactic acid bacteria have been identified, and in this work, six different strains were tested for their ability to produce alpha-amylase and to utilise all sugars with high lactic acid yield in a medium with a complex composition of free sugars (brown juice) and starch. Lactobacillus plantarum A6 was the only strain that showed both a good lactic acid production and utilisation of starch in this medium. The growth rate of this strain was approximately 0.4 h(-1) and the lactic acid yield was 0.7.
    Original languageEnglish
    JournalApplied Microbiology and Biotechnology
    Volume74
    Issue number3
    Pages (from-to)540-546
    ISSN0175-7598
    DOIs
    Publication statusPublished - 2007

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