Basics of antibody phage display technology

Line Ledsgaard, Mogens Kilstrup, Aneesh Karatt-Vellatt, John McCafferty, Andreas Hougaard Laustsen*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Antibody discovery has become increasingly important in almost all areas of modern medicine. Different antibody discovery approaches exist, but one that has gained increasing interest in the field of toxinology and antivenom research is phage display technology. In this review, the lifecycle of the M13 phage and the basics of phage display technology are presented together with important factors influencing the success rates of phage display experiments. Moreover, the pros and cons of different antigen display methods and the use of naïve versus immunized phage display antibody libraries is discussed, and selected examples from the field of antivenom research are highlighted. This review thus provides in-depth knowledge on the principles and use of phage display technology with a special focus on discovery of antibodies that target animal toxins.
Original languageEnglish
Article number236
JournalToxins
Volume10
Issue number6
Number of pages15
ISSN2072-6651
DOIs
Publication statusPublished - 2018

Keywords

  • Antibody discovery
  • Recombinant antivenom
  • Phage display
  • M13 phage
  • Toxinology

Cite this

Ledsgaard, Line ; Kilstrup, Mogens ; Karatt-Vellatt, Aneesh ; McCafferty, John ; Laustsen, Andreas Hougaard. / Basics of antibody phage display technology. In: Toxins. 2018 ; Vol. 10, No. 6.
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author = "Line Ledsgaard and Mogens Kilstrup and Aneesh Karatt-Vellatt and John McCafferty and Laustsen, {Andreas Hougaard}",
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Ledsgaard, L, Kilstrup, M, Karatt-Vellatt, A, McCafferty, J & Laustsen, AH 2018, 'Basics of antibody phage display technology', Toxins, vol. 10, no. 6, 236. https://doi.org/10.3390/toxins10060236

Basics of antibody phage display technology. / Ledsgaard, Line; Kilstrup, Mogens; Karatt-Vellatt, Aneesh; McCafferty, John; Laustsen, Andreas Hougaard.

In: Toxins, Vol. 10, No. 6, 236, 2018.

Research output: Contribution to journalJournal articleResearchpeer-review

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T1 - Basics of antibody phage display technology

AU - Ledsgaard, Line

AU - Kilstrup, Mogens

AU - Karatt-Vellatt, Aneesh

AU - McCafferty, John

AU - Laustsen, Andreas Hougaard

PY - 2018

Y1 - 2018

N2 - Antibody discovery has become increasingly important in almost all areas of modern medicine. Different antibody discovery approaches exist, but one that has gained increasing interest in the field of toxinology and antivenom research is phage display technology. In this review, the lifecycle of the M13 phage and the basics of phage display technology are presented together with important factors influencing the success rates of phage display experiments. Moreover, the pros and cons of different antigen display methods and the use of naïve versus immunized phage display antibody libraries is discussed, and selected examples from the field of antivenom research are highlighted. This review thus provides in-depth knowledge on the principles and use of phage display technology with a special focus on discovery of antibodies that target animal toxins.

AB - Antibody discovery has become increasingly important in almost all areas of modern medicine. Different antibody discovery approaches exist, but one that has gained increasing interest in the field of toxinology and antivenom research is phage display technology. In this review, the lifecycle of the M13 phage and the basics of phage display technology are presented together with important factors influencing the success rates of phage display experiments. Moreover, the pros and cons of different antigen display methods and the use of naïve versus immunized phage display antibody libraries is discussed, and selected examples from the field of antivenom research are highlighted. This review thus provides in-depth knowledge on the principles and use of phage display technology with a special focus on discovery of antibodies that target animal toxins.

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KW - Recombinant antivenom

KW - Phage display

KW - M13 phage

KW - Toxinology

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