Basic and applied uses of genome-scale metabolic network reconstructions of Escherichia coli.

Douglas McCloskey, Bernhard Palsson, Adam Feist

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Abstract

The genome-scale model (GEM) of metabolism in the bacterium Escherichia coli K-12 has been in development for over a decade and is now in wide use. GEM-enabled studies of E. coli have been primarily focused on six applications: (1) metabolic engineering, (2) model-driven discovery, (3) prediction of cellular phenotypes, (4) analysis of biological network properties, (5) studies of evolutionary processes, and (6) models of interspecies interactions. In this review, we provide an overview of these applications along with a critical assessment of their successes and limitations, and a perspective on likely future developments in the field. Taken together, the studies performed over the past decade have established a genome-scale mechanistic understanding of genotype-phenotype relationships in E. coli metabolism that forms the basis for similar efforts for other microbial species. Future challenges include the expansion of GEMs by integrating additional cellular processes beyond metabolism, the identification of key constraints based on emerging data types, and the development of computational methods able to handle such large-scale network models with sufficient accuracy.
Original languageEnglish
JournalMolecular Systems Biology
Volume9
Pages (from-to)661
ISSN1744-4292
DOIs
Publication statusPublished - 2013

Keywords

  • Biological Evolution
  • Computer Simulation
  • Escherichia coli K12
  • Escherichia coli Proteins
  • Gene Expression Regulation, Bacterial
  • Genetic Association Studies
  • Genome, Bacterial
  • Genotype
  • Metabolic Engineering
  • Metabolic Networks and Pathways
  • Models, Genetic
  • Phenotype

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