Barley lipid transfer protein, LTP1, contains a new type of lipid-like post-translational modification

Kresten Lindorff-Larsen, Mathilde Hauge Lerche, Flemming M. Poulsen, Peter Roepstorff, Jakob R. Winther

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

In plants a group of proteins termed nonspecific lipid transfer proteins are found. These proteins bind and catalyze transfer of lipids in vitro, but their in vivo function is unknown. They have been suggested to be involved in different aspects of plant physiology and cell biology, including the formation of cutin and involvement in stress and pathogen responses, but there is yet no direct demonstration of an in vivo function. We have found and characterized a novel post-translational modification of the barley nonspecific lipid transfer protein, LTP1. The protein-modification bond is of a new type in which an aspartic acid in LTP1 is bound to the modification through what most likely is an ester bond. The chemical structure of the modification has been characterized by means of two-dimensional homo- and heteronuclear nuclear magnetic resonance spectroscopy as well as mass spectrometry and is found to be lipid-like in nature. The modification does not resemble any standard lipid post-translational modification but is similar to a compound with known antimicrobial activity.
Original languageEnglish
JournalJournal of Biological Chemistry
Volume276
Issue number36
Pages (from-to)33547-33553
ISSN0021-9258
DOIs
Publication statusPublished - 2001
Externally publishedYes

Keywords

  • Amino Acids
  • Aspartic Acid
  • Carbohydrates
  • Carboxylic Acids
  • Esters
  • Glycosylation
  • Hordeum
  • Hydrogen-Ion Concentration
  • Kinetics
  • Lipids
  • Magnetic Resonance Spectroscopy
  • Mass Spectrometry
  • Models, Chemical
  • Models, Molecular
  • Peptides
  • Plant Proteins
  • Protein Processing, Post-Translational
  • Protein Tyrosine Phosphatases
  • Saccharomyces cerevisiae Proteins
  • Temperature
  • Time Factors
  • Trypsin
  • LTP1 protein, S cerevisiae
  • BIOCHEMISTRY
  • FATTY-ACIDS
  • STRUCTURAL CHARACTERIZATION
  • SPOROTHRIX FLOCCULOSA
  • MASS-SPECTROMETRY
  • BINDING
  • GENE
  • SPECTROSCOPY
  • EXPRESSION
  • FAMILY
  • BEER
  • Biochemistry
  • Agriculture, Biology and Environmental Sciences
  • Engineering and Technology
  • Life Sciences
  • Catalysis
  • Cells
  • Nuclear magnetic resonance spectroscopy
  • Physiology
  • Proteins
  • Antimicrobial activities
  • aspartic acid
  • lipid transfer protein
  • amino acid
  • carbohydrate
  • carboxylic acid
  • ester
  • lipid
  • peptide
  • protein tyrosine phosphatase
  • Saccharomyces cerevisiae protein
  • trypsin
  • vegetable protein
  • antimicrobial activity
  • article
  • barley
  • complex formation
  • disulfide bond
  • hydrophobicity
  • lipid metabolism
  • lipid transport
  • multigene family
  • nonhuman
  • nuclear magnetic resonance
  • priority journal
  • protein lipid interaction
  • protein modification
  • protein processing
  • chemical model
  • chemical structure
  • chemistry
  • glycosylation
  • kinetics
  • mass spectrometry
  • nuclear magnetic resonance spectroscopy
  • pH
  • temperature
  • time
  • Homo
  • Hordeum vulgare subsp. vulgare
  • Protein-Tyrosine-Phosphatase
  • X
  • lipid post-translational modification
  • pathogen responses
  • plant physiology
  • protein-modification bond
  • stress
  • Monocotyledones Angiospermae Spermatophyta Plantae (Angiosperms, Monocots, Plants, Spermatophytes, Vascular Plants) - Gramineae [25305] barley Dansk Landbrugs Grovvareselskab
  • alpha-cyano-4-hydroxycinnamic acid 28166-41-8
  • cutin 54990-88-4
  • lipid transfer protein 1 LTP1
  • 10060, Biochemistry studies - General
  • 51522, Plant physiology - Chemical constituents
  • Bruker AMX600 spectrometer Bruker, laboratory equipment
  • Finnigan Lasermat 2000 time-of-flight spectrometer laboratory equipment
  • mass spectrometry Spectrum Analysis Techniques, analytical method
  • nano-electrospray ionization mass spectrometry Spectrum Analysis Techniques, analytical method
  • NMR spectroscopy Spectrum Analysis Techniques, analytical method, hetero-magnetic, homo-magnetic, two-dimensional
  • Q-TOF mass spectrometer Micromass, laboratory equipment
  • Biochemistry and Molecular Biophysics
  • Equipment, Apparatus, Devices and Instrumentation
  • Methods and Techniques

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