Barley genotypic β-glucan variation combined with enzymatic modifications direct its potential as a natural ingredient in a high fiber extract

Mette S. Mikkelsen, Sebastian Meier, Morten G. Jensen, Fen Qin, Iuliana-Madalina Stoica, Helle J. Martens, Andreas Blennow, Birthe M. Jespersen

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Abstract

β-Glucan was extracted from eight different barley genotypes varying in β-glucan content and molecular structure using Termamyl® SC (T), Attenuzyme® (A) and Attenuzyme® Flex (AF) amylolytic enzymes in combinations. Extracts from barley lines Lys5f, KVL408, KVL1104 and CDC Fibar exceeded 4 g β-glucan/l, providing European Food Safety Authority (EFSA) and U.S. Food and Drug Administration (FDA) recommended amounts (3 g β-glucan/day) from three portions. TAF extracts of Lys5f and KVL408 grains reached extraordinary high concentrations of 8- 9 g β-glucan/l. The β-glucan molecular mass decreased with enzyme treatment T < TA < TAF due to minor lichenase side activity. Extractability was generally higher and molecular mass lower for barley lines low in triosyl/tetraosyl (DP3/DP4) ratios than for those high in DP3/DP4 ratios (Lys5f, KVL408 and KVL1104). Overall, the higher β-glucan content and structural robustness in Lys5f
 and KVL408 raw materials favor these in a β-glucan rich extract with potential for EFSA and FDA health and Nutrition claims.
Original languageEnglish
JournalJournal of Cereal Science
Volume75
Pages (from-to)45-53
ISSN0733-5210
DOIs
Publication statusPublished - 2017

Keywords

  • Barley beta-glucans
  • Extractability
  • Molecular structure
  • Enzymatic hydrolysis

Cite this

Mikkelsen, Mette S. ; Meier, Sebastian ; Jensen, Morten G. ; Qin, Fen ; Stoica, Iuliana-Madalina ; Martens, Helle J. ; Blennow, Andreas ; Jespersen, Birthe M. / Barley genotypic β-glucan variation combined with enzymatic modifications direct its potential as a natural ingredient in a high fiber extract. In: Journal of Cereal Science. 2017 ; Vol. 75. pp. 45-53.
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abstract = "β-Glucan was extracted from eight different barley genotypes varying in β-glucan content and molecular structure using Termamyl{\circledR} SC (T), Attenuzyme{\circledR} (A) and Attenuzyme{\circledR} Flex (AF) amylolytic enzymes in combinations. Extracts from barley lines Lys5f, KVL408, KVL1104 and CDC Fibar exceeded 4 g β-glucan/l, providing European Food Safety Authority (EFSA) and U.S. Food and Drug Administration (FDA) recommended amounts (3 g β-glucan/day) from three portions. TAF extracts of Lys5f and KVL408 grains reached extraordinary high concentrations of 8- 9 g β-glucan/l. The β-glucan molecular mass decreased with enzyme treatment T < TA < TAF due to minor lichenase side activity. Extractability was generally higher and molecular mass lower for barley lines low in triosyl/tetraosyl (DP3/DP4) ratios than for those high in DP3/DP4 ratios (Lys5f, KVL408 and KVL1104). Overall, the higher β-glucan content and structural robustness in Lys5f and KVL408 raw materials favor these in a β-glucan rich extract with potential for EFSA and FDA health and Nutrition claims.",
keywords = "Barley beta-glucans, Extractability, Molecular structure, Enzymatic hydrolysis",
author = "Mikkelsen, {Mette S.} and Sebastian Meier and Jensen, {Morten G.} and Fen Qin and Iuliana-Madalina Stoica and Martens, {Helle J.} and Andreas Blennow and Jespersen, {Birthe M.}",
year = "2017",
doi = "10.1016/j.jcs.2017.02.003",
language = "English",
volume = "75",
pages = "45--53",
journal = "Journal of Cereal Science",
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publisher = "Academic Press",

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Barley genotypic β-glucan variation combined with enzymatic modifications direct its potential as a natural ingredient in a high fiber extract. / Mikkelsen, Mette S.; Meier, Sebastian; Jensen, Morten G.; Qin, Fen; Stoica, Iuliana-Madalina; Martens, Helle J.; Blennow, Andreas; Jespersen, Birthe M.

In: Journal of Cereal Science, Vol. 75, 2017, p. 45-53.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Barley genotypic β-glucan variation combined with enzymatic modifications direct its potential as a natural ingredient in a high fiber extract

AU - Mikkelsen, Mette S.

AU - Meier, Sebastian

AU - Jensen, Morten G.

AU - Qin, Fen

AU - Stoica, Iuliana-Madalina

AU - Martens, Helle J.

AU - Blennow, Andreas

AU - Jespersen, Birthe M.

PY - 2017

Y1 - 2017

N2 - β-Glucan was extracted from eight different barley genotypes varying in β-glucan content and molecular structure using Termamyl® SC (T), Attenuzyme® (A) and Attenuzyme® Flex (AF) amylolytic enzymes in combinations. Extracts from barley lines Lys5f, KVL408, KVL1104 and CDC Fibar exceeded 4 g β-glucan/l, providing European Food Safety Authority (EFSA) and U.S. Food and Drug Administration (FDA) recommended amounts (3 g β-glucan/day) from three portions. TAF extracts of Lys5f and KVL408 grains reached extraordinary high concentrations of 8- 9 g β-glucan/l. The β-glucan molecular mass decreased with enzyme treatment T < TA < TAF due to minor lichenase side activity. Extractability was generally higher and molecular mass lower for barley lines low in triosyl/tetraosyl (DP3/DP4) ratios than for those high in DP3/DP4 ratios (Lys5f, KVL408 and KVL1104). Overall, the higher β-glucan content and structural robustness in Lys5f and KVL408 raw materials favor these in a β-glucan rich extract with potential for EFSA and FDA health and Nutrition claims.

AB - β-Glucan was extracted from eight different barley genotypes varying in β-glucan content and molecular structure using Termamyl® SC (T), Attenuzyme® (A) and Attenuzyme® Flex (AF) amylolytic enzymes in combinations. Extracts from barley lines Lys5f, KVL408, KVL1104 and CDC Fibar exceeded 4 g β-glucan/l, providing European Food Safety Authority (EFSA) and U.S. Food and Drug Administration (FDA) recommended amounts (3 g β-glucan/day) from three portions. TAF extracts of Lys5f and KVL408 grains reached extraordinary high concentrations of 8- 9 g β-glucan/l. The β-glucan molecular mass decreased with enzyme treatment T < TA < TAF due to minor lichenase side activity. Extractability was generally higher and molecular mass lower for barley lines low in triosyl/tetraosyl (DP3/DP4) ratios than for those high in DP3/DP4 ratios (Lys5f, KVL408 and KVL1104). Overall, the higher β-glucan content and structural robustness in Lys5f and KVL408 raw materials favor these in a β-glucan rich extract with potential for EFSA and FDA health and Nutrition claims.

KW - Barley beta-glucans

KW - Extractability

KW - Molecular structure

KW - Enzymatic hydrolysis

U2 - 10.1016/j.jcs.2017.02.003

DO - 10.1016/j.jcs.2017.02.003

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JO - Journal of Cereal Science

JF - Journal of Cereal Science

SN - 0733-5210

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