TY - JOUR
T1 - Bacterial polysaccharide levan as stabilizing, non-toxic and functional coating material for microelement-nanoparticles.
AU - Bondarenko, O. M.
AU - Ivask, A.
AU - Kahru, A.
AU - Vija, H.
AU - Titma, T.
AU - Visnapuu, M.
AU - Joost, U.
AU - Pudova, K.
AU - Adamberg, S.
AU - Visnapuu, Triinu
AU - Alamae, T.
PY - 2016
Y1 - 2016
N2 - Levan, fructose-composed biopolymer of bacterial origin, has potential in biotechnology due to its prebiotic and immunostimulatory properties. In this study levan synthesized by levansucrase from Pseudomonas syringae was thoroughly characterized and used as multifunctional biocompatible coating material for microelement-nanoparticles (NPs) of selenium, iron and cobalt. Transmission electron microscopy (TEM), hydrodynamic size measurements (DLS) and X-ray photoelectron spectroscopy (XPS) showed the interaction of levan with NPs. Levan stabilized the dispersions of NPs, decreased their toxicity and had protective effect on human intestinal cells Caco-2. In addition, levan attached to cobalt NPs remained accessible as a substrate for the colon bacteria Bacteroides thetaiotaomicron. We suggest that the combination of levan and nutritionally important microelements in the form of NPs serves as a first step towards a novel "2 in 1" approach for food supplements to provide safe and efficient delivery of microelements for humans and support beneficial gut microbiota with nutritional oligosaccharides. All rights reserved, Elsevier.
AB - Levan, fructose-composed biopolymer of bacterial origin, has potential in biotechnology due to its prebiotic and immunostimulatory properties. In this study levan synthesized by levansucrase from Pseudomonas syringae was thoroughly characterized and used as multifunctional biocompatible coating material for microelement-nanoparticles (NPs) of selenium, iron and cobalt. Transmission electron microscopy (TEM), hydrodynamic size measurements (DLS) and X-ray photoelectron spectroscopy (XPS) showed the interaction of levan with NPs. Levan stabilized the dispersions of NPs, decreased their toxicity and had protective effect on human intestinal cells Caco-2. In addition, levan attached to cobalt NPs remained accessible as a substrate for the colon bacteria Bacteroides thetaiotaomicron. We suggest that the combination of levan and nutritionally important microelements in the form of NPs serves as a first step towards a novel "2 in 1" approach for food supplements to provide safe and efficient delivery of microelements for humans and support beneficial gut microbiota with nutritional oligosaccharides. All rights reserved, Elsevier.
KW - Safe-by-design
KW - Food supplement
KW - Co, Fe and Se nanomaterial
KW - omaterial Caco-2 in vitro
KW - Bacteroides thetaiotaomicron
U2 - 10.1016/j.carbpol.2015.09.093
DO - 10.1016/j.carbpol.2015.09.093
M3 - Journal article
C2 - 26572404
SN - 0144-8617
VL - 136
SP - 710
EP - 720
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
ER -