Bacterial growth on surfaces: Automated image analysis for quantification of growth rate-related parameters

S. Møller, Claus Sternberg, L. K. Poulsen, Jens Michael Carstensen, Søren Molin

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    A fast routine method for estimating bacterial cell growth rates by using the metachromatic dye acridine orange is described. The method allows simultaneous estimates of cellular RNA and DNA contents of single cells. Acridine orange staining can be used as a nonspecific supplement to quantitative species-specific
    hybridizations with fluorescence-labelled ribosomal probes to estimate the single-cell concentration of RNA. By automated analysis of digitized images of stained cells, we determined four independent growth rate-related parameters: cellular RNA and DNA contents, cell volume, and the frequency of dividing cells in a cell population. These parameters were used to compare physiological states of liquid-suspended and surfacegrowing Pseudomonas putida KT2442 in chemostat cultures. The major finding is that the correlation between substrate availability and cellular growth rate found for the free-living cells was not observed for the surfacebound cells; in contrast, the data indicate an almost constant growth rate for attached cells which was independent of the dilution rate in the chemostat.
    Original languageEnglish
    JournalApplied and Environmental Microbiology
    Volume61
    Issue number2
    Pages (from-to)741-748
    ISSN0099-2240
    Publication statusPublished - 1995

    Fingerprint

    Dive into the research topics of 'Bacterial growth on surfaces: Automated image analysis for quantification of growth rate-related parameters'. Together they form a unique fingerprint.

    Cite this