Backprojection-based reconstruction and correction for distance-dependent defocus in cryoelectron microscopy

Ivan G Kazantsev, Gabor T. Herman, Laslo Cernetic

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    Abstract

    We study two methods of micrograph processing in cryoelectron microscopy: the defocus-gradient corrected back-projection algorithm and the correction of micrographs for the contrast transfer function based on the frequency-distance relation. Analyzing integral images produced by the methods we conclude that they are asymptotically equivalent within the framework of stationary phase approximation.
    Original languageEnglish
    Title of host publicationBiomedical Imaging: From Nano to Macro
    Volume1-4
    Place of PublicationParis
    PublisherIEEE
    Publication date2008
    Pages133-136
    ISBN (Print)14-24-42002-5
    DOIs
    Publication statusPublished - 2008
    Event5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro - Paris, France
    Duration: 14 May 200817 May 2008
    Conference number: 5
    http://ieeexplore.ieee.org/xpl/mostRecentIssue.jsp?punumber=4534844

    Conference

    Conference5th IEEE International Symposium on Biomedical Imaging
    Number5
    CountryFrance
    CityParis
    Period14/05/200817/05/2008
    Internet address

    Bibliographical note

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    Cite this

    Kazantsev, I. G., Herman, G. T., & Cernetic, L. (2008). Backprojection-based reconstruction and correction for distance-dependent defocus in cryoelectron microscopy. In Biomedical Imaging: From Nano to Macro (Vol. 1-4, pp. 133-136). IEEE. https://doi.org/10.1109/ISBI.2008.4540950