Autonomous and 3D real-time multi-beam manipulation in a microfluidic environment

I. Perch-Nielsen, P.J. Rodrigo, C.A. Alonzo, J. Glückstad

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    Abstract

    The Generalized Phase Contrast (GPC) method of optical 3D manipulation has previously been used for controlled spatial manipulation of live biological specimen in real-time. These biological experiments were carried out over a time-span of several hours while an operator intermittently optimized the optical system. Here we present GPC-based optical micromanipulation in a microfluidic system where trapping experiments are computer-automated and thereby capable of running with only limited supervision. The system is able to dynamically detect living yeast cells using a computer-interfaced CCD camera, and respond to this by instantly creating traps at positions of the spotted cells streaming at flow velocities that would be difficult for a human operator to handle. With the added ability to control flow rates, experiments were also carried out to confirm the theoretically predicted axially dependent lateral stiffness of GPC-based optical traps. (c) 2006 Optical Society of America
    Original languageEnglish
    JournalOptics Express
    Volume14
    Issue number25
    Pages (from-to)12199-12205
    ISSN1094-4087
    DOIs
    Publication statusPublished - 11 Dec 2006

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