Automated sperm head morphology analyzer for open-source software

I. A. E. Butts, M. A. R. Ward, M. K. Litvak, T. E. Pitcher, S. M. H. Alavi, E. A. Trippel, R. M. Rideout

Research output: Contribution to journalJournal articleResearchpeer-review


Sperm head morphology has been identified as a characteristic that can be used to predict a male's semen quality. In the present study, we have developed an automated sperm head morphology analysis (ASMA) plug-in for open-source ImageJ software ( We describe the plug-in's functionality, and confirm its validity for sperm head morphology analysis using fish sperm. Sperm head morphological measurements (length and width) made with the ASMA plug-in did not differ from manual measurements. Using the plug-in to measure sperm head-shaped objects of known size, the associated plug-in error rate was <0.5%. Brightness and contrast ratios influenced sperm head measurements, suggesting the need for standardized protocols. This plug-in was effective at measuring elliptical (i.e., Atlantic cod) as well as slightly irregular (i.e., Chinook salmon) shaped sperm heads. In conclusion, our ASMA plug-in represents a versatile alternative to costly sperm morphology software. (C) 2011 Elsevier Inc. All rights reserved.
Original languageEnglish
Issue number9
Pages (from-to)1756-1761
Publication statusPublished - 2011
Externally publishedYes


Dive into the research topics of 'Automated sperm head morphology analyzer for open-source software'. Together they form a unique fingerprint.

Cite this