Automated shape-based clustering of 3D immunoglobulin protein structures in chronic lymphocytic leukemia

Eleftheria Polychronidou*, Ilias Kalamaras, Andreas Agathangelidis, Lesley Ann Sutton, Xiao Jie Yan, Vasilis Bikos, Anna Vardi, Konstantinos Mochament, Nicholas Chiorazzi, Chrysoula Belessi, Richard Rosenquist, Paolo Ghia, Kostas Stamatopoulos, Panayiotis Vlamos, Anna Chailyan, Nanna Overby, Paolo Marcatili, Anastasia Hatzidimitriou, Dimitrios Tzovaras

*Corresponding author for this work

Research output: Contribution to journalConference articleResearchpeer-review

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Abstract

Background: Although the etiology of chronic lymphocytic leukemia (CLL), the most common type of adult leukemia, is still unclear, strong evidence implicates antigen involvement in disease ontogeny and evolution. Primary and 3D structure analysis has been utilised in order to discover indications of antigenic pressure. The latter has been mostly based on the 3D models of the clonotypic B cell receptor immunoglobulin (BcR IG) amino acid sequences. Therefore, their accuracy is directly dependent on the quality of the model construction algorithms and the specific methods used to compare the ensuing models. Thus far, reliable and robust methods that can group the IG 3D models based on their structural characteristics are missing. Results: Here we propose a novel method for clustering a set of proteins based on their 3D structure focusing on 3D structures of BcR IG from a large series of patients with CLL. The method combines techniques from the areas of bioinformatics, 3D object recognition and machine learning. The clustering procedure is based on the extraction of 3D descriptors, encoding various properties of the local and global geometrical structure of the proteins. The descriptors are extracted from aligned pairs of proteins. A combination of individual 3D descriptors is also used as an additional method. The comparison of the automatically generated clusters to manual annotation by experts shows an increased accuracy when using the 3D descriptors compared to plain bioinformatics-based comparison. The accuracy is increased even more when using the combination of 3D descriptors. Conclusions: The experimental results verify that the use of 3D descriptors commonly used for 3D object recognition can be effectively applied to distinguishing structural differences of proteins. The proposed approach can be applied to provide hints for the existence of structural groups in a large set of unannotated BcR IG protein files in both CLL and, by logical extension, other contexts where it is relevant to characterize BcR IG structural similarity. The method does not present any limitations in application and can be extended to other types of proteins.

Original languageEnglish
Article number414
JournalBMC Bioinformatics
Volume19
Issue numberSuppl. 14
Number of pages15
ISSN1471-2105
DOIs
Publication statusPublished - 2018
Event5th International Work-Conference on Bioinformatics and Biomedical Engineering, IWBBIO 2017, Granada, Spain, April 26–28, 2017 - Science Faculty of the University of Granada, Granada, Spain
Duration: 26 Apr 201728 Apr 2017
Conference number: 5
http://iwbbio.ugr.es/

Conference

Conference5th International Work-Conference on Bioinformatics and Biomedical Engineering, IWBBIO 2017, Granada, Spain, April 26–28, 2017
Number5
LocationScience Faculty of the University of Granada
CountrySpain
CityGranada
Period26/04/201728/04/2017
Internet address

Keywords

  • 3D protein descriptors
  • CLL protein clustering
  • Descriptor fusion

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