Automated microfluidic sample-preparation platform for high-throughput structural investigation of proteins by small-angle X-ray scattering

Josiane P. Lafleur, Detlef Snakenborg, Søren Skou Nielsen, Magda Møller, Katrine N. Toft, Andreas Menzel, Jes K. Jacobsen, Bente Vestergaard, Lise Arleth, Jörg Peter Kutter

    Research output: Contribution to journalJournal articleResearchpeer-review

    1423 Downloads (Pure)

    Abstract

    A new microfluidic sample-preparation system is presented for the structural investigation of proteins using small-angle X-ray scattering (SAXS) at synchrotrons. The system includes hardware and software features for precise fluidic control, sample mixing by diffusion, automated X-ray exposure control, UV absorbance measurements and automated data analysis. As little as 15 l of sample is required to perform a complete analysis cycle, including sample mixing, SAXS measurement, continuous UV absorbance measurements, and cleaning of the channels and X-ray cell with buffer. The complete analysis cycle can be performed in less than 3 min. Bovine serum albumin was used as a model protein to characterize the mixing efficiency and sample consumption of the system. The N2 fragment of an adaptor protein (p120-RasGAP) was used to demonstrate how the device can be used to survey the structural space of a protein by screening a wide set of conditions using high-throughput techniques. © 2011 International Union of Crystallography.
    Original languageEnglish
    JournalJournal of Applied Crystallography
    Volume44
    Issue number5
    Pages (from-to)1090-1099
    ISSN0021-8898
    DOIs
    Publication statusPublished - 2011

    Keywords

    • High-throughput
    • UV absorbance
    • Mixing efficiency
    • Automated data analysis
    • Structural analysis
    • Automation
    • Model proteins
    • Hardware and software
    • Data reduction
    • Structural investigation
    • Fluidic control
    • Protein structure
    • Microfluidics
    • Scattering
    • X ray scattering
    • Bovine serum albumins
    • Sample consumption
    • Proteins
    • High-throughput technique
    • Mixing by diffusion
    • Body fluids
    • Small-angle X-ray scattering

    Fingerprint Dive into the research topics of 'Automated microfluidic sample-preparation platform for high-throughput structural investigation of proteins by small-angle X-ray scattering'. Together they form a unique fingerprint.

    Cite this