Authentication of vanillin ex glucose – A first study on the influence of the glucose-source on the δ13C and δ2H value

Amelie Sina Wilde*, Tomas Strucko, Casper Rastgoie Veje, Uffe Hasbro Mortensen, Lene Duedahl-Olesen

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review


Vanillin is the most characteristic compound in vanilla flavour. Since vanilla pods are very expensive, vanillin is mainly produced by synthetic and sometimes biosynthetic methods. Biosynthetic vanillin is called ‘biovanillin’ and can -by law-be labelled as ‘natural’. The isotopic carbon ratio (expressed as δ13C) is a widely established and valuable authentication parameter for vanillin. Furthermore, currently available data about δ2H values indicate, that the isotopic hydrogen ratio can make a decisive contribution to the authentication of vanillin. One of the most recent developments is the bio-production of vanillin derived from glucose. However, very little is known about its isotopic composition. Here, we present the δ13C and δ2H values of glucose from corn and wheat and the vanillin that was derived from these. The isotopic composition of the glucose is clearly reflected in the resulting vanillin molecule. All ‘glucose-vanillin’ samples could be distinguished from vanillin originating from other sources when combining the δ13C and δ2H value.
Original languageEnglish
Article number108389
JournalFood Control
Number of pages8
Publication statusPublished - 2022


  • Authenticity
  • Natural flavor
  • Biovanillin
  • 13C/12C and 2H/1H ratio
  • Glucose
  • Isotope ratio mass spectrometry


Dive into the research topics of 'Authentication of vanillin ex glucose – A first study on the influence of the glucose-source on the δ<sup>13</sup>C and δ<sup>2</sup>H value'. Together they form a unique fingerprint.

Cite this