Attenuating apoptosis in Chinese hamster ovary cells for improved biopharmaceutical production

Matthew N. Henry, Michael A. MacDonald, Camila A. Orellana, Peter P. Gray, Marianne Gillard, Kym Baker, Lars K. Nielsen, Esteban Marcellin, Stephen Mahler, Verónica S. Martínez

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Abstract

Chinese hamster ovary (CHO) cells are the predominant host cell line for the production of biopharmaceuticals, a growing industry currently worth more than $188 billion USD in global sales. CHO cells undergo programmed cell death (apoptosis) following different stresses encountered in cell culture, such as substrate limitation, accumulation of toxic by-products, and mechanical shear, hindering production. Genetic engineering strategies to reduce apoptosis in CHO cells have been investigated with mixed results. In this review, a contemporary understanding of the real complexity of apoptotic mechanisms and signaling pathways is described; followed by an overview of antiapoptotic cell line engineering strategies tested so far in CHO cells.
Original languageEnglish
JournalBiotechnology and Bioengineering
Volume17
Issue number4
Pages (from-to)1187-1203
ISSN0006-3592
DOIs
Publication statusPublished - 2020

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