Attenuating apoptosis in Chinese hamster ovary cells for improved biopharmaceutical production

Matthew N. Henry; Michael A. MacDonald; Camila A. Orellana; Peter P. Gray; Marianne Gillard; Kym Baker; Lars K. Nielsen; Esteban Marcellin; Stephen Mahler; Verónica S. Martínez

doi:10.1002/bit.27269

Attenuating apoptosis in Chinese hamster ovary cells for improved biopharmaceutical production

Matthew N. Henry, Michael A. MacDonald, Camila A. Orellana, Peter P. Gray, Marianne Gillard, Kym Baker, Lars K. Nielsen, Esteban Marcellin, Stephen Mahler, Verónica S. Martínez

University of Queensland

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Abstract

Chinese hamster ovary (CHO) cells are the predominant host cell line for the production of biopharmaceuticals, a growing industry currently worth more than $188 billion USD in global sales. CHO cells undergo programmed cell death (apoptosis) following different stresses encountered in cell culture, such as substrate limitation, accumulation of toxic by-products, and mechanical shear, hindering production. Genetic engineering strategies to reduce apoptosis in CHO cells have been investigated with mixed results. In this review, a contemporary understanding of the real complexity of apoptotic mechanisms and signaling pathways is described; followed by an overview of antiapoptotic cell line engineering strategies tested so far in CHO cells.

Original language	English
Journal	Biotechnology and Bioengineering
Volume	17
Issue number	4
Pages (from-to)	1187-1203
ISSN	0006-3592
DOIs	https://doi.org/10.1002/bit.27269
Publication status	Published - 2020

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title = "Attenuating apoptosis in Chinese hamster ovary cells for improved biopharmaceutical production",

abstract = "Chinese hamster ovary (CHO) cells are the predominant host cell line for the production of biopharmaceuticals, a growing industry currently worth more than $188 billion USD in global sales. CHO cells undergo programmed cell death (apoptosis) following different stresses encountered in cell culture, such as substrate limitation, accumulation of toxic by-products, and mechanical shear, hindering production. Genetic engineering strategies to reduce apoptosis in CHO cells have been investigated with mixed results. In this review, a contemporary understanding of the real complexity of apoptotic mechanisms and signaling pathways is described; followed by an overview of antiapoptotic cell line engineering strategies tested so far in CHO cells.",

author = "Henry, {Matthew N.} and MacDonald, {Michael A.} and Orellana, {Camila A.} and Gray, {Peter P.} and Marianne Gillard and Kym Baker and Nielsen, {Lars K.} and Esteban Marcellin and Stephen Mahler and Mart{\'i}nez, {Ver{\'o}nica S.}",

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AU - Henry, Matthew N.

AU - MacDonald, Michael A.

AU - Orellana, Camila A.

AU - Gray, Peter P.

AU - Gillard, Marianne

AU - Baker, Kym

AU - Nielsen, Lars K.

AU - Marcellin, Esteban

AU - Mahler, Stephen

AU - Martínez, Verónica S.

PY - 2020

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AB - Chinese hamster ovary (CHO) cells are the predominant host cell line for the production of biopharmaceuticals, a growing industry currently worth more than $188 billion USD in global sales. CHO cells undergo programmed cell death (apoptosis) following different stresses encountered in cell culture, such as substrate limitation, accumulation of toxic by-products, and mechanical shear, hindering production. Genetic engineering strategies to reduce apoptosis in CHO cells have been investigated with mixed results. In this review, a contemporary understanding of the real complexity of apoptotic mechanisms and signaling pathways is described; followed by an overview of antiapoptotic cell line engineering strategies tested so far in CHO cells.

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JF - Biotechnology and Bioengineering

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ER -

Attenuating apoptosis in Chinese hamster ovary cells for improved biopharmaceutical production

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