Associations between common intestinal parasites and bacteria in humans as revealed by qPCR

L. O'Brien Andersen, A. B. Karim, Henrik Munch Roager, Louise Kristine Vigsnæs, K. A. Krogfelt, Tine Rask Licht, C. R. Stensvold

Research output: Contribution to journalJournal articleResearchpeer-review


Several studies have shown associations between groups of intestinal bacterial or specific ratios between bacterial groups and various disease traits. Meanwhile, little is known about interactions and associations between eukaryotic and prokaryotic microorganisms in the human gut. In this work, we set out to investigate potential associations between common single-celled parasites such as Blastocystis spp. and Dientamoeba fragilis and intestinal bacteria. Stool DNA from patients with intestinal symptoms were selected based on being Blastocystis spp.-positive (B+)/negative (B-) and D. fragilis-positive (D+)/negative (D-), and split into four groups of 21 samples (B+ D+, B+ D-, B- D+, and B- D-). Quantitative PCR targeting the six bacterial taxa Bacteroides, Prevotella, the butyrate-producing clostridial clusters IV and XIVa, the mucin-degrading Akkermansia muciniphila, and the indigenous group of Bifidobacterium was subsequently performed, and the relative abundance of these bacteria across the four groups was compared. The relative abundance of Bacteroides in B- D- samples was significantly higher compared with B+ D- and B+ D+ samples (P 
Original languageEnglish
JournalEuropean Journal of Clinical Microbiology & Infectious Diseases
Issue number9
Pages (from-to)1427-1431
Number of pages5
Publication statusPublished - 2016


  • Biomedicine
  • Medical Microbiology
  • Internal Medicine
  • SC3


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