Assessment of proteolytic degradation of the basement membrane: a fragment of type IV collagen as a biochemical marker for liver fibrosis

Sanne S. Veidal; Morten A. Karsdal; Arkadiusz Nawrocki; Martin R. Larsen; Yueqin Dai; Qinlong Zheng; Per Hägglund; Ben Vainer; Helene Skjøt-Arkil; Diana J. Leeming

doi:10.1186/1755-1536-4-22

Assessment of proteolytic degradation of the basement membrane: a fragment of type IV collagen as a biochemical marker for liver fibrosis

Sanne S. Veidal, Morten A. Karsdal, Arkadiusz Nawrocki, Martin R. Larsen, Yueqin Dai, Qinlong Zheng, Per Hägglund, Ben Vainer, Helene Skjøt-Arkil, Diana J. Leeming

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Abstract

Collagen deposition and an altered matrix metalloproteinase (MMP) expression profile are hallmarks of fibrosis. Type IV collagen is the most abundant structural basement membrane component of tissue, which increases 14-fold during fibrogenesis in the liver. Proteolytic degradation of collagens by proteases produces small fragments, so-called neoepitopes, which are released systemically. Technologies investigating MMP-generated fragments of collagens may provide more useful information than traditional serological assays that crudely measure total protein. In the present study, we developed an ELISA for the quantification of a neoepitope generated by MMP degradation of type IV collagen and evaluated the association of this neoepitope with liver fibrosis in two animal models.

Original language	English
Journal	Fibrogenesis & Tissue Repair
Volume	4
Issue number	22
Number of pages	11
ISSN	1755-1536
DOIs	https://doi.org/10.1186/1755-1536-4-22
Publication status	Published - 2011

Bibliographical note

© 2011 Veidal et al; licensee BioMed Central Ltd.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Keywords

Biochemical marker
Type IV collagen
Neoepitope
Basement membrane
Extracellular matrix
Liver fibrosis
Protease-cleaved fragment
Matrix metalloproteinase 9

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title = "Assessment of proteolytic degradation of the basement membrane: a fragment of type IV collagen as a biochemical marker for liver fibrosis",

abstract = "Collagen deposition and an altered matrix metalloproteinase (MMP) expression profile are hallmarks of fibrosis. Type IV collagen is the most abundant structural basement membrane component of tissue, which increases 14-fold during fibrogenesis in the liver. Proteolytic degradation of collagens by proteases produces small fragments, so-called neoepitopes, which are released systemically. Technologies investigating MMP-generated fragments of collagens may provide more useful information than traditional serological assays that crudely measure total protein. In the present study, we developed an ELISA for the quantification of a neoepitope generated by MMP degradation of type IV collagen and evaluated the association of this neoepitope with liver fibrosis in two animal models.",

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T1 - Assessment of proteolytic degradation of the basement membrane: a fragment of type IV collagen as a biochemical marker for liver fibrosis

AU - Veidal, Sanne S.

AU - Karsdal, Morten A.

AU - Nawrocki, Arkadiusz

AU - Larsen, Martin R.

AU - Dai, Yueqin

AU - Zheng, Qinlong

AU - Hägglund, Per

AU - Vainer, Ben

AU - Skjøt-Arkil, Helene

AU - Leeming, Diana J.

N1 - © 2011 Veidal et al; licensee BioMed Central Ltd.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PY - 2011

Y1 - 2011

N2 - Collagen deposition and an altered matrix metalloproteinase (MMP) expression profile are hallmarks of fibrosis. Type IV collagen is the most abundant structural basement membrane component of tissue, which increases 14-fold during fibrogenesis in the liver. Proteolytic degradation of collagens by proteases produces small fragments, so-called neoepitopes, which are released systemically. Technologies investigating MMP-generated fragments of collagens may provide more useful information than traditional serological assays that crudely measure total protein. In the present study, we developed an ELISA for the quantification of a neoepitope generated by MMP degradation of type IV collagen and evaluated the association of this neoepitope with liver fibrosis in two animal models.

AB - Collagen deposition and an altered matrix metalloproteinase (MMP) expression profile are hallmarks of fibrosis. Type IV collagen is the most abundant structural basement membrane component of tissue, which increases 14-fold during fibrogenesis in the liver. Proteolytic degradation of collagens by proteases produces small fragments, so-called neoepitopes, which are released systemically. Technologies investigating MMP-generated fragments of collagens may provide more useful information than traditional serological assays that crudely measure total protein. In the present study, we developed an ELISA for the quantification of a neoepitope generated by MMP degradation of type IV collagen and evaluated the association of this neoepitope with liver fibrosis in two animal models.

KW - Biochemical marker

KW - Type IV collagen

KW - Neoepitope

KW - Basement membrane

KW - Extracellular matrix

KW - Liver fibrosis

KW - Protease-cleaved fragment

KW - Matrix metalloproteinase 9

U2 - 10.1186/1755-1536-4-22

DO - 10.1186/1755-1536-4-22

M3 - Journal article

C2 - 21970406

SN - 1755-1536

VL - 4

JO - Fibrogenesis & Tissue Repair

JF - Fibrogenesis & Tissue Repair

IS - 22

ER -

Assessment of proteolytic degradation of the basement membrane: a fragment of type IV collagen as a biochemical marker for liver fibrosis

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Keywords

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