Assessment of proteolytic degradation of the basement membrane: a fragment of type IV collagen as a biochemical marker for liver fibrosis

Sanne S. Veidal, Morten A. Karsdal, Arkadiusz Nawrocki, Martin R. Larsen, Yueqin Dai, Qinlong Zheng, Per Hägglund, Ben Vainer, Helene Skjøt-Arkil, Diana J. Leeming

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    Abstract

    Collagen deposition and an altered matrix metalloproteinase (MMP) expression profile are hallmarks of fibrosis. Type IV collagen is the most abundant structural basement membrane component of tissue, which increases 14-fold during fibrogenesis in the liver. Proteolytic degradation of collagens by proteases produces small fragments, so-called neoepitopes, which are released systemically. Technologies investigating MMP-generated fragments of collagens may provide more useful information than traditional serological assays that crudely measure total protein. In the present study, we developed an ELISA for the quantification of a neoepitope generated by MMP degradation of type IV collagen and evaluated the association of this neoepitope with liver fibrosis in two animal models.
    Original languageEnglish
    JournalFibrogenesis & Tissue Repair
    Volume4
    Issue number22
    Number of pages11
    ISSN1755-1536
    DOIs
    Publication statusPublished - 2011

    Bibliographical note

    © 2011 Veidal et al; licensee BioMed Central Ltd.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

    Keywords

    • Biochemical marker
    • Type IV collagen
    • Neoepitope
    • Basement membrane
    • Extracellular matrix
    • Liver fibrosis
    • Protease-cleaved fragment
    • Matrix metalloproteinase 9

    Cite this

    Veidal, S. S., Karsdal, M. A., Nawrocki, A., Larsen, M. R., Dai, Y., Zheng, Q., Hägglund, P., Vainer, B., Skjøt-Arkil, H., & Leeming, D. J. (2011). Assessment of proteolytic degradation of the basement membrane: a fragment of type IV collagen as a biochemical marker for liver fibrosis. Fibrogenesis & Tissue Repair, 4(22). https://doi.org/10.1186/1755-1536-4-22