Assessment of polycyclic aromatic hydrocarbon exposure, lung function, systemic inflammation, and genotoxicity in peripheral blood mononuclear cells from firefighters before and after a work shift

Maria Helena Guerra Andersen, Anne Thoustrup Saber, Julie Elbæk Pedersen, Peter Bøgh Pedersen, Per Axel Clausen, Mille Løhr, Ali Kermanizadeh, Steffen Loft, Niels E. Ebbeshøj, Åse Marie Hansen, Ismo Kalevi Koponen, Eva Carina Nørskov, Ulla Birgitte Vogel, Peter Møller*

*Corresponding author for this work

    Research output: Contribution to journalJournal articleResearchpeer-review

    63 Downloads (Pure)

    Abstract

    Firefighting is regarded as possibly carcinogenic, although there are few mechanistic studies on genotoxicity in humans. We investigated exposure to polycyclic aromatic hydrocarbons (PAH), lung function, systemic inflammation and genotoxicity in peripheral blood mononuclear cells (PBMC) of 22 professional firefighters before and after a 24-h work shift. Exposure was assessed by measurements of particulate matter (PM), PAH levels on skin, urinary 1-hydroxypyrene (1-OHP) and self-reported participation in fire extinguishing activities. PM measurements indicated that use of personal protective equipment (PPE) effectively prevented inhalation exposure, but exposure to PM occurred when the environment was perceived as safe and the self-contained breathing apparatuses were removed. The level of PAH on skin and urinary 1-OHP concentration were similar before and after the work shift, irrespective of self-reported participation in fire extinction activities. Post-shift, the subjects had reduced levels of oxidatively damaged DNA in PBMC, and increased plasma concentration of vascular cell adhesion molecule 1 (VCAM-1). The subjects reporting participation in fire extinction activities during the work shift had a slightly decreased lung function, increased plasma concentration of VCAM-1, and reduced levels of oxidatively damaged DNA in PBMC. Our results suggest that the firefighters were not exposed to PM while using PPE, but exposure occurred when PPE was not used. The work shift was not associated with increased levels of genotoxicity. Increased levels of VCAM-1 in plasma were observed.
    Original languageEnglish
    JournalEnvironmental and Molecular Mutagenesis
    Volume59
    Issue number6
    Pages (from-to)539-548
    Number of pages10
    ISSN0893-6692
    DOIs
    Publication statusPublished - 2018

    Keywords

    • Biomonitoring
    • Ultrafine particles
    • 1‐hydroxypyrene
    • Oxidative DNA damage
    • Comet assay

    Cite this

    Andersen, M. H. G., Saber, A. T., Pedersen, J. E., Pedersen, P. B., Clausen, P. A., Løhr, M., Kermanizadeh, A., Loft, S., Ebbeshøj, N. E., Hansen, Å. M., Kalevi Koponen, I., Nørskov, E. C., Vogel, U. B., & Møller, P. (2018). Assessment of polycyclic aromatic hydrocarbon exposure, lung function, systemic inflammation, and genotoxicity in peripheral blood mononuclear cells from firefighters before and after a work shift. Environmental and Molecular Mutagenesis, 59(6), 539-548. https://doi.org/10.1002/em.22193