Assessment of flhDC mRNA levels in Serratia liquefaciens swarm cells

Tim Tolker-Nielsen, Allan Beck Christensen, K. Holmstrøm, Leo Eberl, Thomas Bovbjerg Rasmussen, Claus Sternberg, Arne Heydorn, Søren Molin, Michael Christian Givskov

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    Abstract

    We reported previously that artificial overexpression of the flhDC operon in liquid-grown Serratia liquefaciens resulted in the formation of filamentous, multinucleated, and hyperflagellated cells that were indistinguishable from surface-induced swarm cells (L. Eberl, G. Christiansen, S. Molin, and M. Givskov, J. Bacteriol. 178:554-559, 1996). In the present report we show by means of reporter gene measurements, Northern analysis, and in situ reverse transcription-PCR that the amount of flhDC mRNA in surface-grown swarm cells does not exceed the maximum level found in nondifferentiated, vegetative cells. This suggests that surface-induced S. liquefaciens swarm cell differentiation, although dependent on flhDC gene expression, does not occur through elevated flhDC mRNA levels.
    Original languageEnglish
    JournalJournal of Bacteriology
    Volume182
    Issue number10
    Pages (from-to)2680-2686
    ISSN0021-9193
    Publication statusPublished - 2000

    Cite this

    Tolker-Nielsen, T., Christensen, A. B., Holmstrøm, K., Eberl, L., Rasmussen, T. B., Sternberg, C., Heydorn, A., Molin, S., & Givskov, M. C. (2000). Assessment of flhDC mRNA levels in Serratia liquefaciens swarm cells. Journal of Bacteriology, 182(10), 2680-2686.