Assessing the potential of micro-fermentations through screening and media optimisation of recombinant β-carotene production by the yeast Yarrowia lipolytica.

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Abstract

The demand for β-carotene as a natural pigment and an antioxidant has increased in previous years. All projections show that the demand will increase even more in the coming years [1], [2]. Currently β-carotene is produced either chemically or synthetically, the latter has been linked to multiple negative effects including cancer [3]–[7]. These concerns, in addition to a more critical view on synthetic colorants from the public has offset the search for natural or recombinant production platforms. Low productivities and difficulties are some of the issues associated to the few existing naturally-derived production systems [4], [6], [8].
As a result, we chose to focus on the production of recombinant β-carotene from an engineered Yarrowia lipolytica strain [1], and how we could optimise the production capacity as much as possible through media optimisation. In Yarrowia, the terpenoid pathway and lipogenesis share a common precursor, therefore we hypothesised that optimising the lipogenesis would also optimise the synthesis of β-carotene. To maximise the number of parameters in our screening we used the BioLector I micro-fermentation system, which allowed us to run 48 micro-fermentations simultaneously.
Among our findings, we identified parameters to optimise in order to target specific points in the metabolism and determined their optimum values. Through scale-up of experiments from micro-scale to benchtop laboratory-scale bioreactors we determined the scalability of the BioLector system to be accurate. We thereby proved that the BioLector I can be used in studies like ours and in studies were a fast and accurate screening method in needed.
Original languageEnglish
Publication date2019
Number of pages1
Publication statusPublished - 2019
EventXXIX International conference on yeast genetics and molecular biology 2019 - Swedish Exhibition and Congress Centre (Svenska Mässan)/Hotel Gothia Towers, Göteborg, Sweden
Duration: 18 Nov 201922 Nov 2019
Conference number: 29
https://yeast2019.org/

Conference

ConferenceXXIX International conference on yeast genetics and molecular biology 2019
Number29
LocationSwedish Exhibition and Congress Centre (Svenska Mässan)/Hotel Gothia Towers
CountrySweden
CityGöteborg
Period18/11/201922/11/2019
Internet address

Cite this

Jacobsen, I. H., Ledesma-Amaro, R., & Martinez Ruiz, J. L. (2019). Assessing the potential of micro-fermentations through screening and media optimisation of recombinant β-carotene production by the yeast Yarrowia lipolytica.. Abstract from XXIX International conference on yeast genetics and molecular biology 2019, Göteborg, Sweden.