Aspergillus triggers phenazine production in Pseudomonas aeruginosa

Britt Guillaume Jensen, Lars Jelsbak, Ib Søndergaard, Mona Højgaard Pedersen, Jens Christian Frisvad, Kristian Fog Nielsen

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    Abstract

    Objectives: Pseudomonas aeruginosa is an opportunistic human pathogen, commonly infecting cystic fibrosis (CF) patients. Aspergilli, especially Aspergillus fumigatus, are also frequently isolated from CF patients. Our aim was to examine the possible interaction between P. aeruginosa and different Aspergillus species. Methods: A suspension of fungal spores was streaked onto WATM agar plates. After 24 hours incubation at 37 °C, a P. aeruginosa overnight culture was streaked out perpendicular to the fungal streak. The plates were incubated at 37 °C for five days, examined and plugs were extracted for HPLC-DAD and HPLC-DAD-MS analysis. Results: P. aeruginosa PAO1 suppressed growth of A. fumigatus, A. niger, A. flavus, A. oryzae, A. terreus and Emericella nidulans. HPLC and HPLC-DAD-MS results showed an increase in phenazine-1-carboxylic acid and phenazine-1-carboxamide production by P. aeruginosa in the contact area of A. niger, A. flavus, A. oryzae, but not A. fumigatus. In addition, other metabolites with UV chromophores similar to the phenazines were only found in the contact zone between Aspergillus and Pseudomonas. No change in secondary metabolite profiles were seen for the Aspergilli, when comparing with or without the presence of Pseudomonas. Conclusion: All Aspergilli tested, with the exception of A. fumigatus, triggered the upregulation of phenazine-1-carboxamide and phenazine-1-carboxylic acid production by P. aeruginosa. Surprisingly no changes in secondary metabolite profiles were detected in any of the Aspergilli.
    Original languageEnglish
    Publication date2011
    Publication statusPublished - 2011
    EventEuropean Congress on Microbial Biofilms – Basic and Clinical Aspects - Copenhagen, Denmark
    Duration: 1 Jan 2011 → …
    Conference number: 2

    Conference

    ConferenceEuropean Congress on Microbial Biofilms – Basic and Clinical Aspects
    Number2
    CityCopenhagen, Denmark
    Period01/01/2011 → …

    Cite this

    Jensen, B. G., Jelsbak, L., Søndergaard, I., Pedersen, M. H., Frisvad, J. C., & Nielsen, K. F. (2011). Aspergillus triggers phenazine production in Pseudomonas aeruginosa. Abstract from European Congress on Microbial Biofilms – Basic and Clinical Aspects, Copenhagen, Denmark, .