Aspergillus niger contains the cryptic phylogenetic species A. awamori

Giancarlo Perrone, Gaetano Stea, Filomena Epifani, János Varga, Jens Christian Frisvad, Robert A. Samson

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Aspergillus section Nigri is an important group of species for food and medical mycology, and biotechnology. The Aspergillus niger ‘aggregate’ represents its most complicated taxonomic subgroup containing eight morphologically indistinguishable taxa: A. niger, Aspergillus tubingensis, Aspergillus acidus, Aspergillus brasiliensis, Aspergillus costaricaensis, Aspergillus lacticoffeatus, Aspergillus piperis, and Aspergillus vadensis. Aspergillus awamori, first described by Nakazawa, has been compared taxonomically with other black aspergilli and recently it has been treated as a synonym of A. niger. Phylogenetic analyses of sequences generated from portions of three genes coding for the proteins β-tubulin (benA), calmodulin (CaM), and the translation elongation factor-1 alpha (TEF-1α) of a population of A. niger strains isolated from grapes in Europe revealed the presence of a cryptic phylogenetic species within this population, A. awamori. Morphological, physiological, ecological and chemical data overlap occurred between A. niger and the cryptic A. awamori, however the splitting of these two species was also supported by AFLP analysis of the full genome. Isolates in both phylospecies can produce the mycotoxins ochratoxin A and fumonisin B2, and they also share the production of pyranonigrin A, tensidol B, funalenone, malformins, and naphtho-γ-pyrones. In addition, sequence analysis of four putative A. awamori strains from Japan, used in the koji industrial fermentation, revealed that none of these strains belong to the A. awamori phylospecies.
    Original languageEnglish
    JournalFungal Biology
    Volume115
    Issue number11
    Pages (from-to)1138-1150
    ISSN1878-6146
    DOIs
    Publication statusPublished - 2011

    Keywords

    • Aspergillus niger aggregate
    • AFLP
    • Phylogenetic species
    • Multilocus approach
    • Extrolites

    Cite this

    Perrone, Giancarlo ; Stea, Gaetano ; Epifani, Filomena ; Varga, János ; Frisvad, Jens Christian ; Samson, Robert A. / Aspergillus niger contains the cryptic phylogenetic species A. awamori. In: Fungal Biology. 2011 ; Vol. 115, No. 11. pp. 1138-1150.
    @article{6c68fda23b584a36ace7888d7558529a,
    title = "Aspergillus niger contains the cryptic phylogenetic species A. awamori",
    abstract = "Aspergillus section Nigri is an important group of species for food and medical mycology, and biotechnology. The Aspergillus niger ‘aggregate’ represents its most complicated taxonomic subgroup containing eight morphologically indistinguishable taxa: A. niger, Aspergillus tubingensis, Aspergillus acidus, Aspergillus brasiliensis, Aspergillus costaricaensis, Aspergillus lacticoffeatus, Aspergillus piperis, and Aspergillus vadensis. Aspergillus awamori, first described by Nakazawa, has been compared taxonomically with other black aspergilli and recently it has been treated as a synonym of A. niger. Phylogenetic analyses of sequences generated from portions of three genes coding for the proteins β-tubulin (benA), calmodulin (CaM), and the translation elongation factor-1 alpha (TEF-1α) of a population of A. niger strains isolated from grapes in Europe revealed the presence of a cryptic phylogenetic species within this population, A. awamori. Morphological, physiological, ecological and chemical data overlap occurred between A. niger and the cryptic A. awamori, however the splitting of these two species was also supported by AFLP analysis of the full genome. Isolates in both phylospecies can produce the mycotoxins ochratoxin A and fumonisin B2, and they also share the production of pyranonigrin A, tensidol B, funalenone, malformins, and naphtho-γ-pyrones. In addition, sequence analysis of four putative A. awamori strains from Japan, used in the koji industrial fermentation, revealed that none of these strains belong to the A. awamori phylospecies.",
    keywords = "Aspergillus niger aggregate, AFLP, Phylogenetic species, Multilocus approach, Extrolites",
    author = "Giancarlo Perrone and Gaetano Stea and Filomena Epifani and J{\'a}nos Varga and Frisvad, {Jens Christian} and Samson, {Robert A.}",
    year = "2011",
    doi = "10.1016/j.funbio.2011.07.008",
    language = "English",
    volume = "115",
    pages = "1138--1150",
    journal = "Fungal Biology",
    issn = "1878-6146",
    publisher = "Elsevier",
    number = "11",

    }

    Perrone, G, Stea, G, Epifani, F, Varga, J, Frisvad, JC & Samson, RA 2011, 'Aspergillus niger contains the cryptic phylogenetic species A. awamori', Fungal Biology, vol. 115, no. 11, pp. 1138-1150. https://doi.org/10.1016/j.funbio.2011.07.008

    Aspergillus niger contains the cryptic phylogenetic species A. awamori. / Perrone, Giancarlo; Stea, Gaetano; Epifani, Filomena; Varga, János; Frisvad, Jens Christian; Samson, Robert A.

    In: Fungal Biology, Vol. 115, No. 11, 2011, p. 1138-1150.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Aspergillus niger contains the cryptic phylogenetic species A. awamori

    AU - Perrone, Giancarlo

    AU - Stea, Gaetano

    AU - Epifani, Filomena

    AU - Varga, János

    AU - Frisvad, Jens Christian

    AU - Samson, Robert A.

    PY - 2011

    Y1 - 2011

    N2 - Aspergillus section Nigri is an important group of species for food and medical mycology, and biotechnology. The Aspergillus niger ‘aggregate’ represents its most complicated taxonomic subgroup containing eight morphologically indistinguishable taxa: A. niger, Aspergillus tubingensis, Aspergillus acidus, Aspergillus brasiliensis, Aspergillus costaricaensis, Aspergillus lacticoffeatus, Aspergillus piperis, and Aspergillus vadensis. Aspergillus awamori, first described by Nakazawa, has been compared taxonomically with other black aspergilli and recently it has been treated as a synonym of A. niger. Phylogenetic analyses of sequences generated from portions of three genes coding for the proteins β-tubulin (benA), calmodulin (CaM), and the translation elongation factor-1 alpha (TEF-1α) of a population of A. niger strains isolated from grapes in Europe revealed the presence of a cryptic phylogenetic species within this population, A. awamori. Morphological, physiological, ecological and chemical data overlap occurred between A. niger and the cryptic A. awamori, however the splitting of these two species was also supported by AFLP analysis of the full genome. Isolates in both phylospecies can produce the mycotoxins ochratoxin A and fumonisin B2, and they also share the production of pyranonigrin A, tensidol B, funalenone, malformins, and naphtho-γ-pyrones. In addition, sequence analysis of four putative A. awamori strains from Japan, used in the koji industrial fermentation, revealed that none of these strains belong to the A. awamori phylospecies.

    AB - Aspergillus section Nigri is an important group of species for food and medical mycology, and biotechnology. The Aspergillus niger ‘aggregate’ represents its most complicated taxonomic subgroup containing eight morphologically indistinguishable taxa: A. niger, Aspergillus tubingensis, Aspergillus acidus, Aspergillus brasiliensis, Aspergillus costaricaensis, Aspergillus lacticoffeatus, Aspergillus piperis, and Aspergillus vadensis. Aspergillus awamori, first described by Nakazawa, has been compared taxonomically with other black aspergilli and recently it has been treated as a synonym of A. niger. Phylogenetic analyses of sequences generated from portions of three genes coding for the proteins β-tubulin (benA), calmodulin (CaM), and the translation elongation factor-1 alpha (TEF-1α) of a population of A. niger strains isolated from grapes in Europe revealed the presence of a cryptic phylogenetic species within this population, A. awamori. Morphological, physiological, ecological and chemical data overlap occurred between A. niger and the cryptic A. awamori, however the splitting of these two species was also supported by AFLP analysis of the full genome. Isolates in both phylospecies can produce the mycotoxins ochratoxin A and fumonisin B2, and they also share the production of pyranonigrin A, tensidol B, funalenone, malformins, and naphtho-γ-pyrones. In addition, sequence analysis of four putative A. awamori strains from Japan, used in the koji industrial fermentation, revealed that none of these strains belong to the A. awamori phylospecies.

    KW - Aspergillus niger aggregate

    KW - AFLP

    KW - Phylogenetic species

    KW - Multilocus approach

    KW - Extrolites

    U2 - 10.1016/j.funbio.2011.07.008

    DO - 10.1016/j.funbio.2011.07.008

    M3 - Journal article

    VL - 115

    SP - 1138

    EP - 1150

    JO - Fungal Biology

    JF - Fungal Biology

    SN - 1878-6146

    IS - 11

    ER -