Aspergillus hancockii sp. Nov., a biosynthetically talented fungus endemic to southeastern Australian soils

John I. Pitt, Lene Lange, Alastair E. Lacey, Daniel Vuong, David J. Midgley, Paul Greenfield, Mark I. Bradbury, Ernest Lacey, Peter Kamp Busk, Bo Pilgaard, Yit Heng Chooi, Andrew M. Piggott

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Abstract

Aspergillus hancockii sp. nov., classified in Aspergillus subgenus Circumdati section Flavi, was originally isolated from soil in peanut fields near Kumbia, in the South Burnett region of southeast Queensland, Australia, and has since been found occasionally from other substrates and locations in southeast Australia. It is phylogenetically and phenotypically related most closely to A. leporis States and M. Chr., but differs in conidial colour, other minor features and particularly in metabolite profile. When cultivated on rice as an optimal substrate, A. hancockii produced an extensive array of 69 secondary metabolites. Eleven of the 15 most abundant secondary metabolites, constituting 90% of the total area under the curve of the HPLC trace of the crude extract, were novel. The genome of A. hancockii, approximately 40 Mbp, was sequenced and mined for genes encoding carbohydrate degrading enzymes identified the presence of more than 370 genes in 114 gene clusters, demonstrating that A. hancockii has the capacity to degrade cellulose, hemicellulose, lignin, pectin, starch, chitin, cutin and fructan as nutrient sources. Like most Aspergillus species, A. hancockii exhibited a diverse secondary metabolite gene profile, encoding 26 polyketide synthase, 16 nonribosomal peptide synthase and 15 nonribosomal peptide synthase-like enzymes.
Original languageEnglish
Article numbere0170254
JournalPLOS ONE
Volume12
Issue number4
Number of pages21
ISSN1932-6203
DOIs
Publication statusPublished - 2017

Bibliographical note

Copyright: © 2017 Pitt et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Cite this

Pitt, J. I., Lange, L., Lacey, A. E., Vuong, D., Midgley, D. J., Greenfield, P., Bradbury, M. I., Lacey, E., Busk, P. K., Pilgaard, B., Chooi, Y. H., & Piggott, A. M. (2017). Aspergillus hancockii sp. Nov., a biosynthetically talented fungus endemic to southeastern Australian soils. PLOS ONE, 12(4), [e0170254]. https://doi.org/10.1371/journal.pone.0170254