Arsukibacterium ikkense gen. nov., sp nov, a novel alkaliphilic, enzyme-producing gamma-Proteobacterium isolated from a cold and alkaline environment in Greenland

Mariane Schmidt Thøgersen, Anders Prieme, Peter Stougaard

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

A novel aerobic, Gram-negative, non-pigmented bacterium, GCM72T, was isolated from the alkaline, low-saline ikaite columns in the Ikka Fjord, SW Greenland. Strain GCM72T is a motile, non-pigmented, amylase- and protease-producing, oxidase-positive, and catalase-negative bacterium, showing optimal growth Lit pH 9.2-10.0, at 15 degrees C, and at 3% (w/v) NaCl. Major fatty acids were C12:0 3-OH (12.2 ± 0.1%), C16:00 (18.0 ± 0.1%), C18:1 ω7c (10.7 ± 0.5%), and summed feature 3 comprising C16:1ω7c  and/or iso-C15:0 2-OH (36.3 ± 0.7%). Phylogenetic analysis based on 16S rRNA gene sequences showed that isolate GCM72T was most closely related to Rheinheimera baltica and Alishewanella fetalis of the γ-Proteobacteria with a 93% sequence similarity to both. The G + C content of DNA isolated from GCM72T was 49.9 mol% and DNA-DNA hybridization between GCM72T and R. baltica was 9.5%. Fatty acid analysis and G + C content supports a relationship primarily to R. baltica, but several different features, such as a negative catalase-response and optimal growth at low temperature and high pH, together with the large phylogenetic distance and low DNA similarity to its closest relatives, lead us to propose a new genus, Arsukibacterium, gen. nov., with the new species Arsukibacterium ikkense sp. nov. (type strain is GCM72T).
Original languageEnglish
JournalSystematic and Applied Microbiology
Volume30
Issue number3
Pages (from-to)197-201
Number of pages5
ISSN0723-2020
DOIs
Publication statusPublished - 2007
Externally publishedYes

Keywords

  • Applied Microbiology and Biotechnology
  • Proteobacteria
  • Alkaliphilic
  • Arsukibacterium ikkense
  • Enzyme producing
  • Ikaite columns
  • Psychrotolerant
  • Amylase
  • Bacterial DNA
  • Catalase
  • Fatty acid
  • Oxidoreductase
  • Proteinase
  • Sodium chloride
  • Alishewanella fetalis
  • Alkalinity
  • Bacterial growth
  • bacterium isolation
  • Cold
  • Controlled study
  • DNA DNA hybridization
  • DNA isolation
  • Enzyme synthesis
  • Fatty acid analysis
  • Gammaproteobacteria
  • Gene sequence
  • Genus
  • Greenland
  • Microbial activity
  • Nonhuman
  • Nucleotide sequence
  • Phylogeny
  • Priority journal
  • Rheinheimera baltica
  • Sequence homology
  • Strain difference
  • Temperature
  • Aerobiosis
  • DNA, Bacterial
  • DNA, Ribosomal
  • Hydrogen-Ion Concentration
  • Molecular Sequence Data
  • RNA, Ribosomal, 16S
  • Seawater
  • Bacteria (microorganisms)
  • Negibacteria
  • Cold Temperature
  • γ-Proteobacteria
  • Biotechnology
  • Microbiology
  • BIOFILM FORMATION
  • Escherichia-coli
  • Ikaite tufa
  • Water
  • Sea
  • Gamma-Proteobacteria
  • Greenland Nearctic region
  • DNA-DNA hybridization
  • optimal growth
  • Phylogenetic distance
  • Microorganisms (Bacteria, Eubacteria, Microorganisms) - Bacteria [05000*] Arsukibacterium ikkense common new species strain-GCM72 Rheinheimera baltica species Alishewanella fetalis species Arsukibacterium genus new genus
  • 16S rRNA 16S ribosomal RNA
  • Amylase 9000-92-4
  • Catalase 9001-05-2
  • DNA
  • Oxidase 9035-73-8
  • Protease 9001-92-7
  • sodium chloride 7647-14-5
  • 00504, General biology - Taxonomy, nomenclature and terminology
  • 03502, Genetics - General
  • 07502, Ecology: environmental biology - General and methods
  • 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines
  • 10066, Biochemistry studies - Lipids
  • 10802, Enzymes - General and comparative studies: coenzymes
  • 30000, Bacteriology, general and systematic
  • 31000, Physiology and biochemistry of bacteria
  • 31500, Genetics of bacteria and viruses
  • Biochemistry and Molecular Biophysics
  • Population Studies
  • Biogeography
  • Enzymology
  • Molecular Genetics
  • Systematics and Taxonomy

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