Arsukibacterium ikkense gen. nov., sp nov, a novel alkaliphilic, enzyme-producing gamma-Proteobacterium isolated from a cold and alkaline environment in Greenland

Mariane Schmidt Thøgersen; Anders Prieme; Peter Stougaard

doi:10.1016/j.syapm.2006.05.003

Arsukibacterium ikkense gen. nov., sp nov, a novel alkaliphilic, enzyme-producing gamma-Proteobacterium isolated from a cold and alkaline environment in Greenland

Mariane Schmidt Thøgersen, Anders Prieme, Peter Stougaard

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

A novel aerobic, Gram-negative, non-pigmented bacterium, GCM72^T, was isolated from the alkaline, low-saline ikaite columns in the Ikka Fjord, SW Greenland. Strain GCM72^T is a motile, non-pigmented, amylase- and protease-producing, oxidase-positive, and catalase-negative bacterium, showing optimal growth Lit pH 9.2-10.0, at 15 degrees C, and at 3% (w/v) NaCl. Major fatty acids were C_12:0 3-OH (12.2 ± 0.1%), C_16:00 (18.0 ± 0.1%), C_18:1 ω7c (10.7 ± 0.5%), and summed feature 3 comprising C_16:1ω7c and/or iso-C_15:0 2-OH (36.3 ± 0.7%). Phylogenetic analysis based on 16S rRNA gene sequences showed that isolate GCM72^T was most closely related to Rheinheimera baltica and Alishewanella fetalis of the γ-Proteobacteria with a 93% sequence similarity to both. The G + C content of DNA isolated from GCM72^T was 49.9 mol% and DNA-DNA hybridization between GCM72^T and R. baltica was 9.5%. Fatty acid analysis and G + C content supports a relationship primarily to R. baltica, but several different features, such as a negative catalase-response and optimal growth at low temperature and high pH, together with the large phylogenetic distance and low DNA similarity to its closest relatives, lead us to propose a new genus, Arsukibacterium, gen. nov., with the new species Arsukibacterium ikkense sp. nov. (type strain is GCM72^T).

Original language	English
Journal	Systematic and Applied Microbiology
Volume	30
Issue number	3
Pages (from-to)	197-201
Number of pages	5
ISSN	0723-2020
DOIs	https://doi.org/10.1016/j.syapm.2006.05.003
Publication status	Published - 2007
Externally published	Yes

Keywords

Applied Microbiology and Biotechnology
Proteobacteria
Alkaliphilic
Arsukibacterium ikkense
Enzyme producing
Ikaite columns
Psychrotolerant
Amylase
Bacterial DNA
Catalase
Fatty acid
Oxidoreductase
Proteinase
Sodium chloride
Alishewanella fetalis
Alkalinity
Bacterial growth
bacterium isolation
Cold
Controlled study
DNA DNA hybridization
DNA isolation
Enzyme synthesis
Fatty acid analysis
Gammaproteobacteria
Gene sequence
Genus
Greenland
Microbial activity
Nonhuman
Nucleotide sequence
Phylogeny
Priority journal
Rheinheimera baltica
Sequence homology
Strain difference
Temperature
Aerobiosis
DNA, Bacterial
DNA, Ribosomal
Hydrogen-Ion Concentration
Molecular Sequence Data
RNA, Ribosomal, 16S
Seawater
Bacteria (microorganisms)
Negibacteria
Cold Temperature
γ-Proteobacteria
Biotechnology
Microbiology
BIOFILM FORMATION
Escherichia-coli
Ikaite tufa
Water
Sea
Gamma-Proteobacteria
Greenland Nearctic region
DNA-DNA hybridization
optimal growth
Phylogenetic distance
Microorganisms (Bacteria, Eubacteria, Microorganisms) - Bacteria [05000*] Arsukibacterium ikkense common new species strain-GCM72 Rheinheimera baltica species Alishewanella fetalis species Arsukibacterium genus new genus
16S rRNA 16S ribosomal RNA
Amylase 9000-92-4
Catalase 9001-05-2
DNA
Oxidase 9035-73-8
Protease 9001-92-7
sodium chloride 7647-14-5
00504, General biology - Taxonomy, nomenclature and terminology
03502, Genetics - General
07502, Ecology: environmental biology - General and methods
10062, Biochemistry studies - Nucleic acids, purines and pyrimidines
10066, Biochemistry studies - Lipids
10802, Enzymes - General and comparative studies: coenzymes
30000, Bacteriology, general and systematic
31000, Physiology and biochemistry of bacteria
31500, Genetics of bacteria and viruses
Biochemistry and Molecular Biophysics
Population Studies
Biogeography
Enzymology
Molecular Genetics
Systematics and Taxonomy

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@article{b1c551d0f9034323810258a964c89d8d,

title = "Arsukibacterium ikkense gen. nov., sp nov, a novel alkaliphilic, enzyme-producing gamma-Proteobacterium isolated from a cold and alkaline environment in Greenland",

abstract = "A novel aerobic, Gram-negative, non-pigmented bacterium, GCM72T, was isolated from the alkaline, low-saline ikaite columns in the Ikka Fjord, SW Greenland. Strain GCM72T is a motile, non-pigmented, amylase- and protease-producing, oxidase-positive, and catalase-negative bacterium, showing optimal growth Lit pH 9.2-10.0, at 15 degrees C, and at 3% (w/v) NaCl. Major fatty acids were C12:0 3-OH (12.2 ± 0.1%), C16:00 (18.0 ± 0.1%), C18:1 ω7c (10.7 ± 0.5%), and summed feature 3 comprising C16:1ω7c and/or iso-C15:0 2-OH (36.3 ± 0.7%). Phylogenetic analysis based on 16S rRNA gene sequences showed that isolate GCM72T was most closely related to Rheinheimera baltica and Alishewanella fetalis of the γ-Proteobacteria with a 93% sequence similarity to both. The G + C content of DNA isolated from GCM72T was 49.9 mol% and DNA-DNA hybridization between GCM72T and R. baltica was 9.5%. Fatty acid analysis and G + C content supports a relationship primarily to R. baltica, but several different features, such as a negative catalase-response and optimal growth at low temperature and high pH, together with the large phylogenetic distance and low DNA similarity to its closest relatives, lead us to propose a new genus, Arsukibacterium, gen. nov., with the new species Arsukibacterium ikkense sp. nov. (type strain is GCM72T).",

keywords = "Applied Microbiology and Biotechnology, Proteobacteria, Alkaliphilic, Arsukibacterium ikkense, Enzyme producing, Ikaite columns, Psychrotolerant, Amylase, Bacterial DNA, Catalase, Fatty acid, Oxidoreductase, Proteinase, Sodium chloride, Alishewanella fetalis, Alkalinity, Bacterial growth, bacterium isolation, Cold, Controlled study, DNA DNA hybridization, DNA isolation, Enzyme synthesis, Fatty acid analysis, Gammaproteobacteria, Gene sequence, Genus, Greenland, Microbial activity, Nonhuman, Nucleotide sequence, Phylogeny, Priority journal, Rheinheimera baltica, Sequence homology, Strain difference, Temperature, Aerobiosis, DNA, Bacterial, DNA, Ribosomal, Hydrogen-Ion Concentration, Molecular Sequence Data, RNA, Ribosomal, 16S, Seawater, Bacteria (microorganisms), Negibacteria, Cold Temperature, γ-Proteobacteria, Biotechnology, Microbiology, BIOFILM FORMATION, Escherichia-coli, Ikaite tufa, Water, Sea, Gamma-Proteobacteria, Greenland Nearctic region, DNA-DNA hybridization, optimal growth, Phylogenetic distance, Microorganisms (Bacteria, Eubacteria, Microorganisms) - Bacteria [05000*] Arsukibacterium ikkense common new species strain-GCM72 Rheinheimera baltica species Alishewanella fetalis species Arsukibacterium genus new genus, 16S rRNA 16S ribosomal RNA, Amylase 9000-92-4, Catalase 9001-05-2, DNA, Oxidase 9035-73-8, Protease 9001-92-7, sodium chloride 7647-14-5, 00504, General biology - Taxonomy, nomenclature and terminology, 03502, Genetics - General, 07502, Ecology: environmental biology - General and methods, 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines, 10066, Biochemistry studies - Lipids, 10802, Enzymes - General and comparative studies: coenzymes, 30000, Bacteriology, general and systematic, 31000, Physiology and biochemistry of bacteria, 31500, Genetics of bacteria and viruses, Biochemistry and Molecular Biophysics, Population Studies, Biogeography, Enzymology, Molecular Genetics, Systematics and Taxonomy",

author = "{Schmidt Th{\o}gersen}, Mariane and Anders Prieme and Peter Stougaard",

year = "2007",

doi = "10.1016/j.syapm.2006.05.003",

language = "English",

volume = "30",

pages = "197--201",

journal = "Systematic and Applied Microbiology",

issn = "0723-2020",

publisher = "Elsevier",

number = "3",

}

Arsukibacterium ikkense gen. nov., sp nov, a novel alkaliphilic, enzyme-producing gamma-Proteobacterium isolated from a cold and alkaline environment in Greenland. / Schmidt Thøgersen, Mariane; Prieme, Anders; Stougaard, Peter.
In: Systematic and Applied Microbiology, Vol. 30, No. 3, 2007, p. 197-201.

Research output: Contribution to journal › Journal article › Research › peer-review

TY - JOUR

T1 - Arsukibacterium ikkense gen. nov., sp nov, a novel alkaliphilic, enzyme-producing gamma-Proteobacterium isolated from a cold and alkaline environment in Greenland

AU - Schmidt Thøgersen, Mariane

AU - Prieme, Anders

AU - Stougaard, Peter

PY - 2007

Y1 - 2007

N2 - A novel aerobic, Gram-negative, non-pigmented bacterium, GCM72T, was isolated from the alkaline, low-saline ikaite columns in the Ikka Fjord, SW Greenland. Strain GCM72T is a motile, non-pigmented, amylase- and protease-producing, oxidase-positive, and catalase-negative bacterium, showing optimal growth Lit pH 9.2-10.0, at 15 degrees C, and at 3% (w/v) NaCl. Major fatty acids were C12:0 3-OH (12.2 ± 0.1%), C16:00 (18.0 ± 0.1%), C18:1 ω7c (10.7 ± 0.5%), and summed feature 3 comprising C16:1ω7c and/or iso-C15:0 2-OH (36.3 ± 0.7%). Phylogenetic analysis based on 16S rRNA gene sequences showed that isolate GCM72T was most closely related to Rheinheimera baltica and Alishewanella fetalis of the γ-Proteobacteria with a 93% sequence similarity to both. The G + C content of DNA isolated from GCM72T was 49.9 mol% and DNA-DNA hybridization between GCM72T and R. baltica was 9.5%. Fatty acid analysis and G + C content supports a relationship primarily to R. baltica, but several different features, such as a negative catalase-response and optimal growth at low temperature and high pH, together with the large phylogenetic distance and low DNA similarity to its closest relatives, lead us to propose a new genus, Arsukibacterium, gen. nov., with the new species Arsukibacterium ikkense sp. nov. (type strain is GCM72T).

AB - A novel aerobic, Gram-negative, non-pigmented bacterium, GCM72T, was isolated from the alkaline, low-saline ikaite columns in the Ikka Fjord, SW Greenland. Strain GCM72T is a motile, non-pigmented, amylase- and protease-producing, oxidase-positive, and catalase-negative bacterium, showing optimal growth Lit pH 9.2-10.0, at 15 degrees C, and at 3% (w/v) NaCl. Major fatty acids were C12:0 3-OH (12.2 ± 0.1%), C16:00 (18.0 ± 0.1%), C18:1 ω7c (10.7 ± 0.5%), and summed feature 3 comprising C16:1ω7c and/or iso-C15:0 2-OH (36.3 ± 0.7%). Phylogenetic analysis based on 16S rRNA gene sequences showed that isolate GCM72T was most closely related to Rheinheimera baltica and Alishewanella fetalis of the γ-Proteobacteria with a 93% sequence similarity to both. The G + C content of DNA isolated from GCM72T was 49.9 mol% and DNA-DNA hybridization between GCM72T and R. baltica was 9.5%. Fatty acid analysis and G + C content supports a relationship primarily to R. baltica, but several different features, such as a negative catalase-response and optimal growth at low temperature and high pH, together with the large phylogenetic distance and low DNA similarity to its closest relatives, lead us to propose a new genus, Arsukibacterium, gen. nov., with the new species Arsukibacterium ikkense sp. nov. (type strain is GCM72T).

KW - Applied Microbiology and Biotechnology

KW - Proteobacteria

KW - Alkaliphilic

KW - Arsukibacterium ikkense

KW - Enzyme producing

KW - Ikaite columns

KW - Psychrotolerant

KW - Amylase

KW - Bacterial DNA

KW - Catalase

KW - Fatty acid

KW - Oxidoreductase

KW - Proteinase

KW - Sodium chloride

KW - Alishewanella fetalis

KW - Alkalinity

KW - Bacterial growth

KW - bacterium isolation

KW - Cold

KW - Controlled study

KW - DNA DNA hybridization

KW - DNA isolation

KW - Enzyme synthesis

KW - Fatty acid analysis

KW - Gammaproteobacteria

KW - Gene sequence

KW - Genus

KW - Greenland

KW - Microbial activity

KW - Nonhuman

KW - Nucleotide sequence

KW - Phylogeny

KW - Priority journal

KW - Rheinheimera baltica

KW - Sequence homology

KW - Strain difference

KW - Temperature

KW - Aerobiosis

KW - DNA, Bacterial

KW - DNA, Ribosomal

KW - Hydrogen-Ion Concentration

KW - Molecular Sequence Data

KW - RNA, Ribosomal, 16S

KW - Seawater

KW - Bacteria (microorganisms)

KW - Negibacteria

KW - Cold Temperature

KW - γ-Proteobacteria

KW - Biotechnology

KW - Microbiology

KW - BIOFILM FORMATION

KW - Escherichia-coli

KW - Ikaite tufa

KW - Water

KW - Sea

KW - Gamma-Proteobacteria

KW - Greenland Nearctic region

KW - DNA-DNA hybridization

KW - optimal growth

KW - Phylogenetic distance

KW - Microorganisms (Bacteria, Eubacteria, Microorganisms) - Bacteria [05000] Arsukibacterium ikkense common new species strain-GCM72 Rheinheimera baltica species Alishewanella fetalis species Arsukibacterium genus new genus

KW - 16S rRNA 16S ribosomal RNA

KW - Amylase 9000-92-4

KW - Catalase 9001-05-2

KW - DNA

KW - Oxidase 9035-73-8

KW - Protease 9001-92-7

KW - sodium chloride 7647-14-5

KW - 00504, General biology - Taxonomy, nomenclature and terminology

KW - 03502, Genetics - General

KW - 07502, Ecology: environmental biology - General and methods

KW - 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines

KW - 10066, Biochemistry studies - Lipids

KW - 10802, Enzymes - General and comparative studies: coenzymes

KW - 30000, Bacteriology, general and systematic

KW - 31000, Physiology and biochemistry of bacteria

KW - 31500, Genetics of bacteria and viruses

KW - Biochemistry and Molecular Biophysics

KW - Population Studies

KW - Biogeography

KW - Enzymology

KW - Molecular Genetics

KW - Systematics and Taxonomy

U2 - 10.1016/j.syapm.2006.05.003

DO - 10.1016/j.syapm.2006.05.003

M3 - Journal article

C2 - 16790334

SN - 0723-2020

VL - 30

SP - 197

EP - 201

JO - Systematic and Applied Microbiology

JF - Systematic and Applied Microbiology

IS - 3

ER -

Arsukibacterium ikkense gen. nov., sp nov, a novel alkaliphilic, enzyme-producing gamma-Proteobacterium isolated from a cold and alkaline environment in Greenland

Abstract

Keywords

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