Architecture of the E.coli 70S ribosome

N. Burkhardt, G. Diedrich, K.H. Nierhaus, W. Meerwinck, H.B. Stuhrmann, J.S. Pedersen, M.H.J. Koch, V.V. Volkov, M.B. Kozin, D.I. Svergun

    Research output: Contribution to journalJournal articleResearch

    Abstract

    The 70S ribosome from E.coli was analysed by neutron scattering focusing on the shape and the internal protein-RNA-distribution of the complex. Measurements on selectively deuterated 70S particles and free 30S and 50S subunits applying conventional contrast variation and proton-spin contrast-variation resulted in a total of 42 scattering curves. Processing the data on the basis of the spherical harmonic technique, a four-phase model for the 70S ribosome could be generated, which describes the shape of the particle as well as the protein- and the RNA-moieties of each subunit at about 35 Angstrom resolution.
    Original languageEnglish
    JournalPhysica B: Condensed Matter
    Volume234/236
    Pages (from-to)199-201
    ISSN0921-4526
    DOIs
    Publication statusPublished - 1997

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