Abstract
The 70S ribosome from E.coli was analysed by neutron scattering focusing on the shape and the internal protein-RNA-distribution of the complex. Measurements on selectively deuterated 70S particles and free 30S and 50S subunits applying conventional contrast variation and proton-spin contrast-variation resulted in a total of 42 scattering curves. Processing the data on the basis of the spherical harmonic technique, a four-phase model for the 70S ribosome could be generated, which describes the shape of the particle as well as the protein- and the RNA-moieties of each subunit at about 35 Angstrom resolution.
Original language | English |
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Journal | Physica B: Condensed Matter |
Volume | 234/236 |
Pages (from-to) | 199-201 |
ISSN | 0921-4526 |
DOIs | |
Publication status | Published - 1997 |