Viral contamination in waters intended for human consumption or human contact poses a high health riskand can, in worst-case, lead to viral outbreaks. The waterborne virus, norovirus is a major cause of viralgastroenteritis. Conventional detection methods of norovirus rely on microbiological methods likepolymerase chain reaction and a variety of sample preparations. These methods are time consuming,expensive and require highly trained personnel. Thus, viral surveillance cannot be done continuously and only provide an instant overview of the water quality.We are developing an all polymer detection system for online viral surveillance of waters. The detection isbased on differential impedance measurements between a reference and an electrode functionalized with abio-recognition element. The bio-recognition element is an aptamer specific to the target virus. We havepreviously shown very low detection limits with influenza virus as proof of concept of the technology.The electrode material is the intrinsic conducting polymer PEDOT:PSS screen-printed on TOPAS for easyup scaling of production. Finite element simulations of the electrode potential confirm the electrode viabilityin waters with conductivities similar to tap water, see figure 1.Substantial pre-concentration is required toreach the limit of detection needed in surface water. We employ both filter and on-chip based concentrationtechniques to accomplish this. In figure 2, virus presence and successful bio-recognition to the electrodes isestablished as an initial test. On-chip concentration is done by electric focusing of the virus particles. Fromextensive finite element modelling, we have designed dielectrophoresis channels with embeddedmicroelectrodes to focus the virus particles in the center and thus facilitate concentration of the particles.
|Title of host publication||Abstract Book - DTU Sustain Conference 2014|
|Number of pages||1|
|Place of Publication||Kgs. Lyngby|
|Publisher||Technical University of Denmark|
|Publication status||Published - 2014|
|Event||DTU Sustain Conference 2014 - Technical University of Denmark, Lyngby, Denmark|
Duration: 17 Dec 2014 → 17 Dec 2014
|Conference||DTU Sustain Conference 2014|
|Location||Technical University of Denmark|
|Period||17/12/2014 → 17/12/2014|
Rozlosnik, N., Kirkegaard, J., Olsen, M. H., Dimaki, M., & Svendsen, W. E. (2014). AquaVir- Portable Analyzer for Water Borne Infectious Viruses. In Abstract Book - DTU Sustain Conference 2014 Technical University of Denmark.