Applications of ribosomal in situ hybridization for the study of bacterial cells in the mouse intestine

Tine Rask Licht, Lars Kongsbak Poulsen, Søren Molin, Karen Angeliki Krogfelt

Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review


Localization of E. coli and S. typhimurium in the large and small intestine of streptomycin-treated mice was visualized by in situ hybridization with specific rRNA target probes and epi-fluorescence microscopy. Growth rates of E. coli BJ4 colonizing the large intestine of streptomycin-treated mice were estimated by quantitative hybridization. The ribosomal contents were measured in bacteria isolated from cecal mucus, cecal contents and feces and correlated with the ribosomal contents of bacteria growing in vitro with defined rates. The data suggest that E. coli BJ4 grows with an apparent doubling time of 40-80 minutes in the intestinal mucosa.
Original languageEnglish
Title of host publicationEcology of pathogenic bacteria ; Molecular and evolutionary aspects
Place of PublicationAmsterdam
PublisherRoyal Netherlands Academy of Arts and Sciences
Publication date1997
Publication statusPublished - 1997
EventEcology of pathogenic bacteria; Molecular and evolutionary aspects - Amsterdam
Duration: 1 Jan 1995 → …


ConferenceEcology of pathogenic bacteria; Molecular and evolutionary aspects
Period01/01/1995 → …

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