Abstract
The paper describes a method of simultaneous determination of the
external and the solid phase mass-transfer coefficients from
frontal analysis data. The protein flux to the solid particles is
determined from the slope of the breakthrough curve and the
mass-transfer coefficients are determined by fitting the two film
model to the experimentally determined flux. The two film model is
compared with two apparent over-all driving force models: The
apparent over-all mobile phase driving force model and the
apparent over-all solid phase driving force model. The experiments
show that the apparent over-all driving force models fail to
describe the flux correctly and this is substantiated by the
theory. Results obtained with BSA on the anion exchange media Q
HyperD, Source, and Poros show that the external film resistance
is significant for Reynolds numbers less than one. The
experimental Sherwood numbers are lower than expected and their
dependence on the Reynolds number are much higher than expected.
Original language | English |
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Journal | J. Chromatography A |
Volume | 827 |
Pages (from-to) | 259-267 |
Publication status | Published - 1999 |