Abstract
| Original language | English |
|---|---|
| Title of host publication | Host-Pathogen Interactions |
| Editors | Francisco Javier López-Baena, Carlos Medina |
| Number of pages | 28 |
| Volume | 1734 |
| Publisher | Springer |
| Publication date | 2017 |
| Pages | 131-158 |
| ISBN (Print) | 978-1-4939-7603-4 |
| ISBN (Electronic) | 978-1-4939-7604-1 |
| DOIs | |
| Publication status | Published - 2017 |
| Series | Methods in Molecular Biology |
|---|---|
| ISSN | 1064-3745 |
Keywords
- Bacterial pathogens
- Bacterial physiology
- Biofilms
- Interspecies interactions
- Microbe–microbe interactions
- RNA sequencing
Cite this
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Application of RNA-seq and Bioimaging Methods to Study Microbe-Microbe Interactions and Their Effects on Biofilm Formation and Gene Expression. / Amador Hierro, Cristina Isabel; Sternberg, Claus; Jelsbak, Lars.
Host-Pathogen Interactions. ed. / Francisco Javier López-Baena; Carlos Medina. Vol. 1734 Springer, 2017. p. 131-158 (Methods in Molecular Biology).Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
TY - CHAP
T1 - Application of RNA-seq and Bioimaging Methods to Study Microbe-Microbe Interactions and Their Effects on Biofilm Formation and Gene Expression
AU - Amador Hierro, Cristina Isabel
AU - Sternberg, Claus
AU - Jelsbak, Lars
PY - 2017
Y1 - 2017
N2 - Complex interactions between pathogenic bacteria, the microbiota, and the host can modify pathogen physiology and behavior. We describe two different experimental approaches to study microbe-microbe interactions in in vitro systems containing surface-associated microbial populations. One method is the application of RNA sequencing (RNA-seq) to determine the transcriptional changes in pathogenic bacteria in response to microbial interspecies interactions. The other method combines flow cell devices for bacterial cultivation and growth with high-resolution bioimaging to analyze the microscale structural organization of interacting microbial populations within mixed-species biofilms.
AB - Complex interactions between pathogenic bacteria, the microbiota, and the host can modify pathogen physiology and behavior. We describe two different experimental approaches to study microbe-microbe interactions in in vitro systems containing surface-associated microbial populations. One method is the application of RNA sequencing (RNA-seq) to determine the transcriptional changes in pathogenic bacteria in response to microbial interspecies interactions. The other method combines flow cell devices for bacterial cultivation and growth with high-resolution bioimaging to analyze the microscale structural organization of interacting microbial populations within mixed-species biofilms.
KW - Bacterial pathogens
KW - Bacterial physiology
KW - Biofilms
KW - Interspecies interactions
KW - Microbe–microbe interactions
KW - RNA sequencing
U2 - 10.1007/978-1-4939-7604-1_12
DO - 10.1007/978-1-4939-7604-1_12
M3 - Book chapter
SN - 978-1-4939-7603-4
VL - 1734
SP - 131
EP - 158
BT - Host-Pathogen Interactions
A2 - López-Baena, Francisco Javier
A2 - Medina, Carlos
PB - Springer
ER -