@inbook{eff891aaee714d26898ba3585387886f,
title = "Application of RNA-seq and Bioimaging Methods to Study Microbe-Microbe Interactions and Their Effects on Biofilm Formation and Gene Expression",
abstract = "Complex interactions between pathogenic bacteria, the microbiota, and the host can modify pathogen physiology and behavior. We describe two different experimental approaches to study microbe-microbe interactions in in vitro systems containing surface-associated microbial populations. One method is the application of RNA sequencing (RNA-seq) to determine the transcriptional changes in pathogenic bacteria in response to microbial interspecies interactions. The other method combines flow cell devices for bacterial cultivation and growth with high-resolution bioimaging to analyze the microscale structural organization of interacting microbial populations within mixed-species biofilms.",
keywords = "Bacterial pathogens, Bacterial physiology, Biofilms, Interspecies interactions, Microbe–microbe interactions, RNA sequencing",
author = "\{Amador Hierro\}, \{Cristina Isabel\} and Claus Sternberg and Lars Jelsbak",
year = "2017",
doi = "10.1007/978-1-4939-7604-1\_12",
language = "English",
isbn = "978-1-4939-7603-4",
volume = "1734",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "131--158",
editor = "L{\'o}pez-Baena, \{Francisco Javier\} and Carlos Medina",
booktitle = "Host-Pathogen Interactions",
}