Application of RNA-seq and Bioimaging Methods to Study Microbe-Microbe Interactions and Their Effects on Biofilm Formation and Gene Expression

Cristina Isabel Amador Hierro; Claus Sternberg; Lars Jelsbak

doi:10.1007/978-1-4939-7604-1_12

Application of RNA-seq and Bioimaging Methods to Study Microbe-Microbe Interactions and Their Effects on Biofilm Formation and Gene Expression

Cristina Isabel Amador Hierro, Claus Sternberg, Lars Jelsbak

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Abstract

Complex interactions between pathogenic bacteria, the microbiota, and the host can modify pathogen physiology and behavior. We describe two different experimental approaches to study microbe-microbe interactions in in vitro systems containing surface-associated microbial populations. One method is the application of RNA sequencing (RNA-seq) to determine the transcriptional changes in pathogenic bacteria in response to microbial interspecies interactions. The other method combines flow cell devices for bacterial cultivation and growth with high-resolution bioimaging to analyze the microscale structural organization of interacting microbial populations within mixed-species biofilms.

Original language	English
Title of host publication	Host-Pathogen Interactions
Editors	Francisco Javier López-Baena, Carlos Medina
Number of pages	28
Volume	1734
Publisher	Springer
Publication date	2017
Pages	131-158
ISBN (Print)	978-1-4939-7603-4
ISBN (Electronic)	978-1-4939-7604-1
DOIs	https://doi.org/10.1007/978-1-4939-7604-1_12
Publication status	Published - 2017

Series	Methods in Molecular Biology
ISSN	1064-3745

Keywords

Bacterial pathogens
Bacterial physiology
Biofilms
Interspecies interactions
Microbe–microbe interactions
RNA sequencing

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10.1007/978-1-4939-7604-1_12

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Amador Hierro, C. I., Sternberg, C., & Jelsbak, L. (2017). Application of RNA-seq and Bioimaging Methods to Study Microbe-Microbe Interactions and Their Effects on Biofilm Formation and Gene Expression. In F. J. López-Baena, & C. Medina (Eds.), Host-Pathogen Interactions (Vol. 1734, pp. 131-158). Springer. Methods in Molecular Biology https://doi.org/10.1007/978-1-4939-7604-1_12

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abstract = "Complex interactions between pathogenic bacteria, the microbiota, and the host can modify pathogen physiology and behavior. We describe two different experimental approaches to study microbe-microbe interactions in in vitro systems containing surface-associated microbial populations. One method is the application of RNA sequencing (RNA-seq) to determine the transcriptional changes in pathogenic bacteria in response to microbial interspecies interactions. The other method combines flow cell devices for bacterial cultivation and growth with high-resolution bioimaging to analyze the microscale structural organization of interacting microbial populations within mixed-species biofilms.",

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Application of RNA-seq and Bioimaging Methods to Study Microbe-Microbe Interactions and Their Effects on Biofilm Formation and Gene Expression. / Amador Hierro, Cristina Isabel; Sternberg, Claus ; Jelsbak, Lars.

Host-Pathogen Interactions. ed. / Francisco Javier López-Baena; Carlos Medina. Vol. 1734 Springer, 2017. p. 131-158 (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

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