Application of RNA-seq and Bioimaging Methods to Study Microbe-Microbe Interactions and Their Effects on Biofilm Formation and Gene Expression

Cristina Isabel Amador Hierro, Claus Sternberg, Lars Jelsbak

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Abstract

Complex interactions between pathogenic bacteria, the microbiota, and the host can modify pathogen physiology and behavior. We describe two different experimental approaches to study microbe-microbe interactions in in vitro systems containing surface-associated microbial populations. One method is the application of RNA sequencing (RNA-seq) to determine the transcriptional changes in pathogenic bacteria in response to microbial interspecies interactions. The other method combines flow cell devices for bacterial cultivation and growth with high-resolution bioimaging to analyze the microscale structural organization of interacting microbial populations within mixed-species biofilms.
Original languageEnglish
Title of host publicationHost-Pathogen Interactions
EditorsFrancisco Javier López-Baena, Carlos Medina
Number of pages28
Volume1734
PublisherSpringer
Publication date2017
Pages131-158
ISBN (Print)978-1-4939-7603-4
ISBN (Electronic)978-1-4939-7604-1
DOIs
Publication statusPublished - 2017
SeriesMethods in Molecular Biology
ISSN1064-3745

Keywords

  • Bacterial pathogens
  • Bacterial physiology
  • Biofilms
  • Interspecies interactions
  • Microbe–microbe interactions
  • RNA sequencing

Cite this

Amador Hierro, C. I., Sternberg, C., & Jelsbak, L. (2017). Application of RNA-seq and Bioimaging Methods to Study Microbe-Microbe Interactions and Their Effects on Biofilm Formation and Gene Expression. In F. J. López-Baena, & C. Medina (Eds.), Host-Pathogen Interactions (Vol. 1734, pp. 131-158). Springer. Methods in Molecular Biology https://doi.org/10.1007/978-1-4939-7604-1_12