Application of quercetin and its bio-inspired nanoparticles as anti-adhesive agents against Bacillus subtilis attachment to surface.

Diana S. Raie, Eisha Mhatre, Matthias Thiele, A. Labena, Gamal El-Ghannam, Laila A. Farahat, Tareq Youssef, Wolfgang Fritzsche, Ákos T. Kovács

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The aim of this study was directed to reveal the repulsive effect of coated glass slides by quercetin and its bio-inspired titanium oxide and tungsten oxide nanoparticles on physical surface attachment of Bacillus subtilis as an ab-initio step of biofilm formation. Nanoparticles were successfully synthesized using sol–gel and acid precipitation methods for titanium oxide and tungsten oxide, respectively (in the absence or presence of quercetin). The anti-adhesive impact of the coated-slides was tested through the physical attachment of B. subtilis after 24 h using Confocal Laser Scanning Microscopy (CLSM). Here, quercetin was presented as a bio-route for the synthesis of tungsten mixed oxides nano-plates at room temperature. In addition, quercetin had an impact on zeta potential and adsorption capacity of both bio-inspired amorphous titanium oxide and tungsten oxide nano-plates. Interestingly, our experiments indicated a contrary effect of quercetin as an anti-adhesive agent than previously reported. However, its bio-inspired metal oxide proved their repulsive efficiency. In addition, quercetin-mediated nano-tungsten and quercetin-mediated amorphous titanium showed anti-adhesive activity against B. subtilis biofilm.
Original languageEnglish
JournalMaterials Science and Engineering C: Materials for Biological Applications
Volume70
Pages (from-to)753-762
DOIs
Publication statusPublished - 2017
Externally publishedYes

Keywords

  • Quercetin
  • Nanoparticles
  • Bacillus subtilis
  • Biofilm
  • Metal oxide
  • Anti-adhesion

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