Application of interpretable artificial neural networks to early monoclonal antibodies development

Lorenzo Gentiluomo*, Dierk Roessner, Dillen Augustijn, Hristo Svilenov, Alina Kulakova, Sujata Mahapatra, Gerhard Winter, Werner Streicher, Åsmund Rinnan, Günther H.J. Peters, Pernille Harris, Wolfgang Frieß

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The development of a new protein drug typically starts with the design, expression and biophysical characterization of many different protein constructs. The initially high number of constructs is radically reduced to a few candidates that exhibit the desired biological and physicochemical properties. This process of protein expression and characterization to find the most promising molecules is both expensive and time-consuming. Consequently, many companies adopt and implement philosophies, e.g. platforms for protein expression and formulation, computational approaches, machine learning, to save resources and facilitate protein drug development. Inspired by this, we propose the use of interpretable artificial neuronal networks (ANNs) to predict biophysical properties of therapeutic monoclonal antibodies i.e. melting temperature Tm, aggregation onset temperature Tagg, interaction parameter kD as a function of pH and salt concentration from the amino acid composition. Our ANNs were trained with typical early-stage screening datasets achieving high prediction accuracy. By only using the amino acid composition, we could keep the ANNs simple which allows for high general applicability, robustness and interpretability. Finally, we propose a novel "knowledge transfer" approach, which can be readily applied due to the simple algorithm design, to understand how our ANNs come to their conclusions.
Original languageEnglish
JournalEuropean Journal of Pharmaceutics and Biopharmaceutics
Volume141
Pages (from-to)81-89
Number of pages9
ISSN0939-6411
DOIs
Publication statusPublished - 2019

Keywords

  • Machine learning
  • Monoclonal antibody
  • Neural network(s)
  • Protein aggregation
  • Protein formulation
  • Stability

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