Application of fluorescent in situ hybridization for specific diagnosis of Pneumocystis carinii pneumonia in foals and pigs

Tim Kåre Jensen, Mette Boye, Vivi Bille-Hansen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Fluorescent in situ hybridization, immunohistochemistry, and Grocott's methenamine-silver nitrate staining were compared as diagnostic methods for Pneumocystis carinii pneumonia in formalin-fixed lung tissue from foals and pigs. An oligonucleotide probe targeting 18S ribosomal RNA of P, carinii was designed for in situ hybridization, and a commercially available monoclonal antibody was used for immunohistochemistry. Samples from six foals and 10 pigs with P. carinii pneumonia, as verified by Grocott's methenamine-silver nitrate staining:, were examined concurrently with samples from seven animals with pneumonia caused by other pathogens. Fluorescent in situ hybridization showed distinctive positive reactions for P. carinii in all test samples. The immunohistochemical procedure, however, only revealed P. carinii in the foals. The number of P. carinii organisms observed by fluorescent in situ hybridization and immunohistochemistry far exceeded the number of organisms stained by Grocott's methenamine-silver nitrate staining. The results show that fluorescent in situ hybridization targeting ribosomal RNA can provide a specific diagnosis of P. carinii pneumonia in foals and pigs.
    Original languageEnglish
    JournalVeterinary Pathology
    Volume38
    Issue number3
    Pages (from-to)269-274
    ISSN0300-9858
    DOIs
    Publication statusPublished - 2001

    Fingerprint

    Dive into the research topics of 'Application of fluorescent in situ hybridization for specific diagnosis of Pneumocystis carinii pneumonia in foals and pigs'. Together they form a unique fingerprint.

    Cite this