Application of fluorescent in situ hybridisation for demonstration of Coxiella burnetti in placentas from ruminant abortions

Tim Kåre Jensen; Donald L. Montgomery; Paula T. Jaeger; Tina Lindhardt; Jørgen Agerholm; Vivi Bille-Hansen; Mette Boye

doi:10.1111/j.1600-0463.2007.apm_591.x

Application of fluorescent in situ hybridisation for demonstration of Coxiella burnetti in placentas from ruminant abortions

Tim Kåre Jensen, Donald L. Montgomery, Paula T. Jaeger, Tina Lindhardt, Jørgen Agerholm, Vivi Bille-Hansen, Mette Boye

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

A fluorescent in situ hybridisation (FISH) assay targeting 16S ribosomal RNA was developed for detection of the zoonotic bacterium Coxiella burnetii in formalin-fixed, paraffin-embedded tissue, and applied on placentas from ruminant abortions. The applicability of the FISH assay was compared to immunohistochemistry (IHC) using human positive control serum in 12 cases of C burnetii-associated placentitis as well as 7 negative control tissue samples. In all 12 cases the bacterium was detected within trophoblasts as well as free in the placental debris by both FISH and IHC. Extensive and significant infection by C. burnetii was revealed in 10 of the cases, whereas a slighter and focal distribution of the bacterium was observed in two cases. 90 aborted placentas from Danish ruminants were investigated by FISH. C burnetii was detected in one bovine case only, representing the first confirmation of C burnetii in Danish animals. The study shows that FISH targeting 16S ribosomal RNA is a feasible diagnostic tool for detection of C burnetii in tissue samples and fully comparable to IHC.

Original language	English
Journal	Acta Pathologica Microbiologica et Immunologica Scandinavica
Volume	115
Issue number	4
Pages (from-to)	347-353
ISSN	0903-4641
DOIs	https://doi.org/10.1111/j.1600-0463.2007.apm_591.x
Publication status	Published - 2007

Keywords

Coxiella burnetii
immunohistochemistry
in situ hybridisation

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Jensen, T. K., Montgomery, D. L., Jaeger, P. T., Lindhardt, T., Agerholm, J., Bille-Hansen, V., & Boye, M. (2007). Application of fluorescent in situ hybridisation for demonstration of Coxiella burnetti in placentas from ruminant abortions. Acta Pathologica Microbiologica et Immunologica Scandinavica, 115(4), 347-353. https://doi.org/10.1111/j.1600-0463.2007.apm_591.x

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abstract = "A fluorescent in situ hybridisation (FISH) assay targeting 16S ribosomal RNA was developed for detection of the zoonotic bacterium Coxiella burnetii in formalin-fixed, paraffin-embedded tissue, and applied on placentas from ruminant abortions. The applicability of the FISH assay was compared to immunohistochemistry (IHC) using human positive control serum in 12 cases of C burnetii-associated placentitis as well as 7 negative control tissue samples. In all 12 cases the bacterium was detected within trophoblasts as well as free in the placental debris by both FISH and IHC. Extensive and significant infection by C. burnetii was revealed in 10 of the cases, whereas a slighter and focal distribution of the bacterium was observed in two cases. 90 aborted placentas from Danish ruminants were investigated by FISH. C burnetii was detected in one bovine case only, representing the first confirmation of C burnetii in Danish animals. The study shows that FISH targeting 16S ribosomal RNA is a feasible diagnostic tool for detection of C burnetii in tissue samples and fully comparable to IHC.",

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Application of fluorescent in situ hybridisation for demonstration of Coxiella burnetti in placentas from ruminant abortions. / Jensen, Tim Kåre; Montgomery, Donald L.; Jaeger, Paula T. et al.
In: Acta Pathologica Microbiologica et Immunologica Scandinavica, Vol. 115, No. 4, 2007, p. 347-353.

Research output: Contribution to journal › Journal article › Research › peer-review

TY - JOUR

T1 - Application of fluorescent in situ hybridisation for demonstration of Coxiella burnetti in placentas from ruminant abortions

AU - Jensen, Tim Kåre

AU - Montgomery, Donald L.

AU - Jaeger, Paula T.

AU - Lindhardt, Tina

AU - Agerholm, Jørgen

AU - Bille-Hansen, Vivi

AU - Boye, Mette

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N2 - A fluorescent in situ hybridisation (FISH) assay targeting 16S ribosomal RNA was developed for detection of the zoonotic bacterium Coxiella burnetii in formalin-fixed, paraffin-embedded tissue, and applied on placentas from ruminant abortions. The applicability of the FISH assay was compared to immunohistochemistry (IHC) using human positive control serum in 12 cases of C burnetii-associated placentitis as well as 7 negative control tissue samples. In all 12 cases the bacterium was detected within trophoblasts as well as free in the placental debris by both FISH and IHC. Extensive and significant infection by C. burnetii was revealed in 10 of the cases, whereas a slighter and focal distribution of the bacterium was observed in two cases. 90 aborted placentas from Danish ruminants were investigated by FISH. C burnetii was detected in one bovine case only, representing the first confirmation of C burnetii in Danish animals. The study shows that FISH targeting 16S ribosomal RNA is a feasible diagnostic tool for detection of C burnetii in tissue samples and fully comparable to IHC.

AB - A fluorescent in situ hybridisation (FISH) assay targeting 16S ribosomal RNA was developed for detection of the zoonotic bacterium Coxiella burnetii in formalin-fixed, paraffin-embedded tissue, and applied on placentas from ruminant abortions. The applicability of the FISH assay was compared to immunohistochemistry (IHC) using human positive control serum in 12 cases of C burnetii-associated placentitis as well as 7 negative control tissue samples. In all 12 cases the bacterium was detected within trophoblasts as well as free in the placental debris by both FISH and IHC. Extensive and significant infection by C. burnetii was revealed in 10 of the cases, whereas a slighter and focal distribution of the bacterium was observed in two cases. 90 aborted placentas from Danish ruminants were investigated by FISH. C burnetii was detected in one bovine case only, representing the first confirmation of C burnetii in Danish animals. The study shows that FISH targeting 16S ribosomal RNA is a feasible diagnostic tool for detection of C burnetii in tissue samples and fully comparable to IHC.

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KW - immunohistochemistry

KW - in situ hybridisation

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JO - Acta Pathologica Microbiologica et Immunologica Scandinavica

JF - Acta Pathologica Microbiologica et Immunologica Scandinavica

IS - 4

ER -

Application of fluorescent in situ hybridisation for demonstration of Coxiella burnetti in placentas from ruminant abortions

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Keywords

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