Apparatus and method for a fully automated preparation of a hyperpolarizing imaging agent

Provided is an apparatus and method for automated hyperpolarization of samples for use as an imaging agent comprising a sample box, an airlock chamber configured to receive a sample from the sample box, a cryogenic chamber, a guide channel to transport samples from the airlock chamber to the cryogenic chamber, a heater and pressure module coupled to the sample box, an insertion and retraction device to transport samples through the guide channel to and from the cryogenic chamber, a dissolution module coupled to the sample box, and a controller to regulate hyperpolarization of the samples by controlling one or more of position, sequencing, temperature, pressure, and dissolution of the samples within the apparatus. Also provided is a machine-readable medium comprising instruction which, when executed by a controller, causes a hyperpolarization apparatus to perform the steps of hyperpolarization of a sample.