Antibody Specific B-Cell Epitope Predictions: Leveraging Information From Antibody-Antigen Protein Complexes

Martin Closter Jespersen, Swapnil Mahajan, Bjoern Peters, Morten Nielsen*, Paolo Marcatili

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

B-cells can neutralize pathogenic molecules by targeting them with extreme specificity using receptors secreted or expressed on their surface (antibodies). This is achieved via molecular interactions between the paratope (i.e., the antibody residues involved in the binding) and the interacting region (epitope) of its target molecule (antigen). Discerning the rules that define this specificity would have profound implications for our understanding of humoral immunogenicity and its applications. The aim of this work is to produce improved, antibody-specific epitope predictions by exploiting features derived from the antigens and their cognate antibodies structures, and combining them using statistical and machine learning algorithms. We have identified several geometric and physicochemical features that are correlated in interacting paratopes and epitopes, used them to develop a Monte Carlo algorithm to generate putative epitopes-paratope pairs, and train a machine-learning model to score them. We show that, by including the structural and physicochemical properties of the paratope, we improve the prediction of the target of a given B-cell receptor. Moreover, we demonstrate a gain in predictive power both in terms of identifying the cognate antigen target for a given antibody and the antibody target for a given antigen, exceeding the results of other available tools.

Original languageEnglish
Article number298
JournalFrontiers in Immunology
Volume10
Number of pages10
ISSN1664-3224
DOIs
Publication statusPublished - 2019

Keywords

  • Antigen
  • Antibody
  • B cell epitope
  • Prediction
  • Paratope
  • Antibody specific epitope prediction

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