Antibody Cross-Reactivity in Antivenom Research

Line Ledsgaard, Timothy P Jenkins, Kristian Davidsen, Kamille Elvstrøm Krause, Andrea Martos-Esteban, Mikael Engmark, Mikael Rørdam Andersen, Ole Lund, Andreas Hougaard Laustsen*

*Corresponding author for this work

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Abstract

Antivenom cross-reactivity has been investigated for decades to determine which antivenoms can be used to treat snakebite envenomings from different snake species. Traditionally, the methods used for analyzing cross-reactivity have been immunodiffusion, immunoblotting, enzyme-linked immunosorbent assay (ELISA), enzymatic assays, and in vivo neutralization studies. In recent years, new methods for determination of cross-reactivity have emerged, including surface plasmon resonance, antivenomics, and high-density peptide microarray technology. Antivenomics involves a top-down assessment of the toxin-binding capacities of antivenoms, whereas high-density peptide microarray technology may be harnessed to provide in-depth knowledge on which toxin epitopes are recognized by antivenoms. This review provides an overview of both the classical and new methods used to investigate antivenom cross-reactivity, the advantages and disadvantages of each method, and examples of studies using the methods. A special focus is given to antivenomics and high-density peptide microarray technology as these high-throughput methods have recently been introduced in this field and may enable more detailed assessments of antivenom cross-reactivity.
Original languageEnglish
Article number393
JournalToxins
Volume10
Issue number10
Number of pages19
ISSN2072-6651
DOIs
Publication statusPublished - 2018

Bibliographical note

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

Keywords

  • Antivenom
  • Cross-reactivity
  • Cross-neutralization
  • High-density peptide microarray technology
  • Antivenomics
  • Snakebite envenoming
  • Venom
  • Toxins

Cite this

Ledsgaard, L., Jenkins, T. P., Davidsen, K., Krause, K. E., Martos-Esteban, A., Engmark, M., ... Laustsen, A. H. (2018). Antibody Cross-Reactivity in Antivenom Research. Toxins, 10(10), [393]. https://doi.org/10.3390/toxins10100393
Ledsgaard, Line ; Jenkins, Timothy P ; Davidsen, Kristian ; Krause, Kamille Elvstrøm ; Martos-Esteban, Andrea ; Engmark, Mikael ; Andersen, Mikael Rørdam ; Lund, Ole ; Laustsen, Andreas Hougaard. / Antibody Cross-Reactivity in Antivenom Research. In: Toxins. 2018 ; Vol. 10, No. 10.
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abstract = "Antivenom cross-reactivity has been investigated for decades to determine which antivenoms can be used to treat snakebite envenomings from different snake species. Traditionally, the methods used for analyzing cross-reactivity have been immunodiffusion, immunoblotting, enzyme-linked immunosorbent assay (ELISA), enzymatic assays, and in vivo neutralization studies. In recent years, new methods for determination of cross-reactivity have emerged, including surface plasmon resonance, antivenomics, and high-density peptide microarray technology. Antivenomics involves a top-down assessment of the toxin-binding capacities of antivenoms, whereas high-density peptide microarray technology may be harnessed to provide in-depth knowledge on which toxin epitopes are recognized by antivenoms. This review provides an overview of both the classical and new methods used to investigate antivenom cross-reactivity, the advantages and disadvantages of each method, and examples of studies using the methods. A special focus is given to antivenomics and high-density peptide microarray technology as these high-throughput methods have recently been introduced in this field and may enable more detailed assessments of antivenom cross-reactivity.",
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Ledsgaard, L, Jenkins, TP, Davidsen, K, Krause, KE, Martos-Esteban, A, Engmark, M, Andersen, MR, Lund, O & Laustsen, AH 2018, 'Antibody Cross-Reactivity in Antivenom Research', Toxins, vol. 10, no. 10, 393. https://doi.org/10.3390/toxins10100393

Antibody Cross-Reactivity in Antivenom Research. / Ledsgaard, Line; Jenkins, Timothy P; Davidsen, Kristian; Krause, Kamille Elvstrøm; Martos-Esteban, Andrea; Engmark, Mikael; Andersen, Mikael Rørdam; Lund, Ole; Laustsen, Andreas Hougaard.

In: Toxins, Vol. 10, No. 10, 393, 2018.

Research output: Contribution to journalJournal articleResearchpeer-review

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T1 - Antibody Cross-Reactivity in Antivenom Research

AU - Ledsgaard, Line

AU - Jenkins, Timothy P

AU - Davidsen, Kristian

AU - Krause, Kamille Elvstrøm

AU - Martos-Esteban, Andrea

AU - Engmark, Mikael

AU - Andersen, Mikael Rørdam

AU - Lund, Ole

AU - Laustsen, Andreas Hougaard

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N2 - Antivenom cross-reactivity has been investigated for decades to determine which antivenoms can be used to treat snakebite envenomings from different snake species. Traditionally, the methods used for analyzing cross-reactivity have been immunodiffusion, immunoblotting, enzyme-linked immunosorbent assay (ELISA), enzymatic assays, and in vivo neutralization studies. In recent years, new methods for determination of cross-reactivity have emerged, including surface plasmon resonance, antivenomics, and high-density peptide microarray technology. Antivenomics involves a top-down assessment of the toxin-binding capacities of antivenoms, whereas high-density peptide microarray technology may be harnessed to provide in-depth knowledge on which toxin epitopes are recognized by antivenoms. This review provides an overview of both the classical and new methods used to investigate antivenom cross-reactivity, the advantages and disadvantages of each method, and examples of studies using the methods. A special focus is given to antivenomics and high-density peptide microarray technology as these high-throughput methods have recently been introduced in this field and may enable more detailed assessments of antivenom cross-reactivity.

AB - Antivenom cross-reactivity has been investigated for decades to determine which antivenoms can be used to treat snakebite envenomings from different snake species. Traditionally, the methods used for analyzing cross-reactivity have been immunodiffusion, immunoblotting, enzyme-linked immunosorbent assay (ELISA), enzymatic assays, and in vivo neutralization studies. In recent years, new methods for determination of cross-reactivity have emerged, including surface plasmon resonance, antivenomics, and high-density peptide microarray technology. Antivenomics involves a top-down assessment of the toxin-binding capacities of antivenoms, whereas high-density peptide microarray technology may be harnessed to provide in-depth knowledge on which toxin epitopes are recognized by antivenoms. This review provides an overview of both the classical and new methods used to investigate antivenom cross-reactivity, the advantages and disadvantages of each method, and examples of studies using the methods. A special focus is given to antivenomics and high-density peptide microarray technology as these high-throughput methods have recently been introduced in this field and may enable more detailed assessments of antivenom cross-reactivity.

KW - Antivenom

KW - Cross-reactivity

KW - Cross-neutralization

KW - High-density peptide microarray technology

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KW - Snakebite envenoming

KW - Venom

KW - Toxins

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DO - 10.3390/toxins10100393

M3 - Journal article

VL - 10

JO - Toxins

JF - Toxins

SN - 2072-6651

IS - 10

M1 - 393

ER -

Ledsgaard L, Jenkins TP, Davidsen K, Krause KE, Martos-Esteban A, Engmark M et al. Antibody Cross-Reactivity in Antivenom Research. Toxins. 2018;10(10). 393. https://doi.org/10.3390/toxins10100393