Antibacterial activity and mutagenesis of sponge-associated Pseudomonas fluorescens H41

Lumeng Ye, Juliana F. Santos-Gandelman, Cristiane C. P. Hardoim, Isabelle George, Pierre Cornelis, Marinella S. Laport

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Marine sponges (phylum Porifera) are well known to harbour a complex and diverse bacterial community. Some of these sponge-associated bacteria have been shown to be the real producers of secondary metabolites with a wide range of activities from antimicrobials to anticancer agents. Previously, we revealed that the strain Pseudomonas fluorescens H41 isolated from the sponge Haliclona sp. (collected at the coast of Rio de Janeiro, Brazil) showed a strong antimicrobial activity against clinical and marine bacteria. Thus, in this study the genes involved in the antimicrobial activity of P. fluorescens H41 were identified. To this end, a library of mutants was generated via miniTnphoA3 transposon mutagenesis and the resulting clones were characterized for their antimicrobial activity. It was demonstrated that genes involved in the biosynthesis of the pyoverdine siderophore are related to the inhibitory activity of P. fluorescens H41. Therefore, this strain might play an important role in the biocontrol of the host sponge.
Original languageEnglish
JournalAntonie van Leeuwenhoek: Journal of Microbiology
Volume108
Issue number1
Pages (from-to)117-126
ISSN0003-6072
DOIs
Publication statusPublished - 2015
Externally publishedYes

Keywords

  • Antagonism
  • Antimicrobial
  • Pseudomonas fluorescens
  • Pyoverdine
  • Porifera
  • Brazil nuts

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