Ancient pathogen DNA in human teeth and petrous bones

Research output: Contribution to journalJournal article – Annual report year: 2018Researchpeer-review

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  • Author: Margaryan, Ashot

    University of Copenhagen, Denmark

  • Author: Hansen, Henrik B.

    University of Copenhagen, Denmark

  • Author: Rasmussen, Simon

    Metagenomics, Department of Bio and Health Informatics, Technical University of Denmark, Kemitorvet, 2800, Kgs. Lyngby, Denmark

  • Author: Sikora, Martin

    University of Copenhagen, Denmark

  • Author: Moiseyev, Vyacheslav

    Peter the Great Museum of Anthropology and Ethnography, Russian Federation

  • Author: Khoklov, Alexandr

    Samara State University, Russian Federation

  • Author: Epimakhov, Andrey

    South Ural State University, Russian Federation

  • Author: Yepiskoposyan, Levon

    National Academy of Sciences of the Republic of Armenia, Armenia

  • Author: Kriiska, Aivar

    Tallinn University, Estonia

  • Author: Varul, Liivi

    Tallinn University, Estonia

  • Author: Saag, Lehti

    University of Tartu, Estonia

  • Author: Lynnerup, Niels

    University of Copenhagen, Denmark

  • Author: Willerslev, Eske

    University of Copenhagen, Denmark

  • Author: Allentoft, Morten E.

    University of Copenhagen, Denmark

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Recent ancient DNA (aDNA) studies of human pathogens have provided invaluable insights into their evolutionary history and prevalence in space and time. Most of these studies were based on DNA extracted from teeth or postcranial bones. In contrast, no pathogen DNA has been reported from the petrous bone which has become the most desired skeletal element in ancient DNA research due to its high endogenous DNA content. To compare the potential for pathogenic aDNA retrieval from teeth and petrous bones, we sampled these elements from five ancient skeletons, previously shown to be carrying Yersinia pestis. Based on shotgun sequencing data, four of these five plague victims showed clearly detectable levels of Y.pestis DNA in the teeth, whereas all the petrous bones failed to produce Y.pestis DNA above baseline levels. A broader comparative metagenomic analysis of teeth and petrous bones from 10 historical skeletons corroborated these results, showing a much higher microbial diversity in teeth than petrous bones, including pathogenic and oral microbial taxa. Our results imply that although petrous bones are highly valuable for ancient genomic analyses as an excellent source of endogenous DNA, the metagenomic potential of these dense skeletal elements is highly limited. This trade-off must be considered when designing the sampling strategy for an aDNA project.
Original languageEnglish
JournalEcology and Evolution
Volume8
Issue number6
Pages (from-to)3534-3542
Number of pages9
ISSN2045-7758
DOIs
Publication statusPublished - 2018

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This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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