Analytical tools for unravelling the metabolism of gas-fermenting Clostridia

James K. Heffernan; Vishnu Mahamkali; Kaspar Valgepea; Esteban Marcellin; Lars K. Nielsen

doi:10.1016/j.copbio.2022.102700

Analytical tools for unravelling the metabolism of gas-fermenting Clostridia

James K. Heffernan, Vishnu Mahamkali, Kaspar Valgepea, Esteban Marcellin, Lars K. Nielsen^*

^*Corresponding author for this work

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Abstract

Acetogens harness the Wood-Ljungdahl Pathway, a unique metabolic pathway for C1 capture close to the thermodynamic limit. Gas fermentation using acetogens is already used for CO-to-ethanol conversion at industrial-scale and has the potential to valorise a range of C1 and waste substrates to short-chain and medium-chain carboxylic acids and alcohols. Advances in analytical quantification and metabolic modelling have helped guide industrial gas fermentation designs. Further advances in the measurements of difficult to measure metabolites are required to improve kinetic modelling and understand the regulation of acetogen metabolism. This will help guide future synthetic biology designs needed to realise the full potential of gas fermentation in stimulating a circular bioeconomy.

Original language	English
Article number	102700
Journal	Current Opinion in Biotechnology
Volume	75
Number of pages	10
ISSN	0958-1669
DOIs	https://doi.org/10.1016/j.copbio.2022.102700
Publication status	Published - 2022

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title = "Analytical tools for unravelling the metabolism of gas-fermenting Clostridia",

abstract = "Acetogens harness the Wood-Ljungdahl Pathway, a unique metabolic pathway for C1 capture close to the thermodynamic limit. Gas fermentation using acetogens is already used for CO-to-ethanol conversion at industrial-scale and has the potential to valorise a range of C1 and waste substrates to short-chain and medium-chain carboxylic acids and alcohols. Advances in analytical quantification and metabolic modelling have helped guide industrial gas fermentation designs. Further advances in the measurements of difficult to measure metabolites are required to improve kinetic modelling and understand the regulation of acetogen metabolism. This will help guide future synthetic biology designs needed to realise the full potential of gas fermentation in stimulating a circular bioeconomy.",

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AU - Marcellin, Esteban

AU - Nielsen, Lars K.

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AB - Acetogens harness the Wood-Ljungdahl Pathway, a unique metabolic pathway for C1 capture close to the thermodynamic limit. Gas fermentation using acetogens is already used for CO-to-ethanol conversion at industrial-scale and has the potential to valorise a range of C1 and waste substrates to short-chain and medium-chain carboxylic acids and alcohols. Advances in analytical quantification and metabolic modelling have helped guide industrial gas fermentation designs. Further advances in the measurements of difficult to measure metabolites are required to improve kinetic modelling and understand the regulation of acetogen metabolism. This will help guide future synthetic biology designs needed to realise the full potential of gas fermentation in stimulating a circular bioeconomy.

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JO - Current Opinion in Biotechnology

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Analytical tools for unravelling the metabolism of gas-fermenting Clostridia

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