Analysis of vitamin K-1 in fruits and vegetables using accelerated solvent extraction and liquid chromatography tandem mass spectrometry with atmospheric pressure chemical ionization

Rie Bak Jäpelt, Jette Jakobsen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The objective of this study was to develop a rapid, sensitive, and specific analytical method to study vitamin K-1 in fruits and vegetables. Accelerated solvent extraction and solid phase extraction was used for sample preparation. Quantification was done by liquid chromatography tandem mass spectrometry with atmospheric pressure chemical ionization in selected reaction monitoring mode with deuterium-labeled vitamin K1 as an internal standard. The precision was estimated as the pooled estimate of three replicates performed on three different days for spinach, peas, apples, banana, and beetroot. The repeatability was 5.2% and the internal reproducibility was 6.2%. Recovery was in the range 90-120%. No significant difference was observed between the results obtained by the present method and by a method using the same principle as the CEN-standard i.e. liquid-liquid extraction and post-column zinc reduction with fluorescence detection. Limit of quantification was estimated to 0.05 mu g/100 g fresh weight. (C) 2015 Elsevier Ltd. All rights reserved.
Original languageEnglish
JournalFood Chemistry
Volume192
Pages (from-to)402-408
Number of pages7
ISSN0308-8146
DOIs
Publication statusPublished - 2016

Keywords

  • Vitamin K-1
  • Fruits and vegetables
  • Accelerated solvent extraction
  • LC-MS/MS
  • APCI

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