Analysis of Vibrio vulnificus from market oysters and septicemia cases for virulence markers

A DePaola, JL Nordstrom, A. Dalsgaard, Anita Forslund, J Oliver, T Bates, KL Bourdage, PA Gulig

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Representative encapsulated strains of Vibrio vulnificus from market oysters and oyster-associated primary septicemia cases (25 isolates each) were tested in a blinded fashion for potential virulence markers that may distinguish strains from these two sources. These isolates were analyzed for plasmid content, for the presence of a 460-bp amplicon by randomly amplified polymorphic DNA PCR, and for virulence in subcutaneously (s.c.) inoculated, iron-dextran-treated mice. Similar percentages of market oyster and clinical isolates possessed detectable plasmids (24 and 36%, respectively), produced the 460-bp amplicon (45 and 50%, respectively), and were judged to be virulent in the mouse s.c. inoculation-iron-dextran model (88% for each). Therefore, it appears that nearly all V vulnificus strains in oysters are virulent and that genetic tests for plasmids and specific PCR size amplicons cannot distinguish between fully virulent and less virulent strains or between clinical and environmental isolates. The inability of these methods to distinguish food and clinical V. vulnificus isolates demonstrates the need for alternative subtyping approaches and virulence assays.
Original languageEnglish
JournalAPPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume69
Issue number7
Pages (from-to)4006-4011
Number of pages6
ISSN0099-2240
DOIs
Publication statusPublished - 2003
Externally publishedYes

Keywords

  • BIOTECHNOLOGY
  • MICROBIOLOGY
  • FIELD GEL-ELECTROPHORESIS
  • UNITED-STATES
  • PLASMID DNA
  • STRAINS
  • PATHOGENESIS
  • EPIDEMIOLOGY
  • INFECTIONS
  • RETAIL
  • COAST
  • Environmental Science (all)
  • Biotechnology
  • Microbiology
  • Disease control
  • DNA
  • Iron
  • Clinical isolates
  • Bacteria
  • dextran
  • iron
  • virulence
  • amplicon
  • animal experiment
  • animal model
  • article
  • bacterial strain
  • bacterial virulence
  • bacterium identification
  • controlled study
  • mouse
  • nonhuman
  • oyster
  • plasmid
  • polymerase chain reaction
  • random amplified polymorphic DNA
  • septicemia
  • Vibrio vulnificus
  • Animals
  • Bacteremia
  • Colony Count, Microbial
  • Disease Models, Animal
  • DNA, Bacterial
  • Humans
  • Iron-Dextran Complex
  • Mice
  • Ostreidae
  • Plasmids
  • Polymerase Chain Reaction
  • Random Amplified Polymorphic DNA Technique
  • Vibrio Infections
  • Virulence
  • Animalia
  • Bacteria (microorganisms)
  • Negibacteria
  • Vibrio

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