Analysis of the Degradome with the CLIP-CHIP™ Microarray

Reinhild Kappelhoff, Ulrich auf dem Keller, Christopher M. Overall

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Abstract

The degradome microarray – CLIP-CHIP™ – is a dedicated and focused array that allows the analysis of all proteases, non-proteolytic homologs, and protease inhibitor gene transcripts in the human and murine genomes at the mRNA transcript level.

Based on unique 70-mer oligonucleotides, designed to match parts of the sequence of known or predicted protease and inhibitor mRNAs in both species and printed on a glass-matrix surface, the CLIP-CHIP™ microarray can be used to analyze differentially expressed protease and inhibitor gene products and give expression profiles for any analyzed sample.
Original languageEnglish
Title of host publicationMatrix Metalloproteinase Protocols
PublisherSpringer
Publication date2010
Pages175-193
DOIs
Publication statusPublished - 2010
Externally publishedYes

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