Analysis of several irdoid and indole precursors of terpenoid indole alkaloids with a single HPLC run.

Denise Dagnino; Jan Schripsema; Robert Verpoorte

doi:10.1055/s-2006-957879

Analysis of several irdoid and indole precursors of terpenoid indole alkaloids with a single HPLC run.

Denise Dagnino
, Jan Schripsema
, Robert Verpoorte

Leiden University

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

An isocratic HPLC system is described which allows the separation of the iridoid and indole precursors of terpenoid indole alkaloids, which are present in a single crude extract. The system consists of a column of LiChrospher 60 RP select B 5 μm, 250 × 4mm (Merck) with an eluent of 1% formic acid-acetonitrile-trichloroacetic acid (100: 10: 0.25, v: v: w) at a flow of 1.2ml/min. In the suspension cultures of Catharanthus roseus secologanin and tryptophan were detected. In the cultures of Tabernaemontana divaricata loganin, tryptophan, and tryptamine accumulated.

Original language	English
Journal	Planta Medica
Volume	62
Issue number	3
Pages (from-to)	278-280
ISSN	0032-0943
DOIs	https://doi.org/10.1055/s-2006-957879
Publication status	Published - 1996

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title = "Analysis of several irdoid and indole precursors of terpenoid indole alkaloids with a single HPLC run.",

abstract = "An isocratic HPLC system is described which allows the separation of the iridoid and indole precursors of terpenoid indole alkaloids, which are present in a single crude extract. The system consists of a column of LiChrospher 60 RP select B 5 μm, 250 × 4mm (Merck) with an eluent of 1\% formic acid-acetonitrile-trichloroacetic acid (100: 10: 0.25, v: v: w) at a flow of 1.2ml/min. In the suspension cultures of Catharanthus roseus secologanin and tryptophan were detected. In the cultures of Tabernaemontana divaricata loganin, tryptophan, and tryptamine accumulated.",

author = "Denise Dagnino and Jan Schripsema and Robert Verpoorte",

year = "1996",

doi = "10.1055/s-2006-957879",

language = "English",

volume = "62",

pages = "278--280",

journal = "Planta Medica",

issn = "0032-0943",

publisher = "Georg Thieme Verlag",

number = "3",

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TY - JOUR

T1 - Analysis of several irdoid and indole precursors of terpenoid indole alkaloids with a single HPLC run.

AU - Dagnino, Denise

AU - Schripsema, Jan

AU - Verpoorte, Robert

PY - 1996

Y1 - 1996

N2 - An isocratic HPLC system is described which allows the separation of the iridoid and indole precursors of terpenoid indole alkaloids, which are present in a single crude extract. The system consists of a column of LiChrospher 60 RP select B 5 μm, 250 × 4mm (Merck) with an eluent of 1% formic acid-acetonitrile-trichloroacetic acid (100: 10: 0.25, v: v: w) at a flow of 1.2ml/min. In the suspension cultures of Catharanthus roseus secologanin and tryptophan were detected. In the cultures of Tabernaemontana divaricata loganin, tryptophan, and tryptamine accumulated.

AB - An isocratic HPLC system is described which allows the separation of the iridoid and indole precursors of terpenoid indole alkaloids, which are present in a single crude extract. The system consists of a column of LiChrospher 60 RP select B 5 μm, 250 × 4mm (Merck) with an eluent of 1% formic acid-acetonitrile-trichloroacetic acid (100: 10: 0.25, v: v: w) at a flow of 1.2ml/min. In the suspension cultures of Catharanthus roseus secologanin and tryptophan were detected. In the cultures of Tabernaemontana divaricata loganin, tryptophan, and tryptamine accumulated.

U2 - 10.1055/s-2006-957879

DO - 10.1055/s-2006-957879

M3 - Journal article

SN - 0032-0943

VL - 62

SP - 278

EP - 280

JO - Planta Medica

JF - Planta Medica

IS - 3

ER -

Analysis of several irdoid and indole precursors of terpenoid indole alkaloids with a single HPLC run.

Abstract

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