Abstract
An isocratic HPLC system is described which allows the separation
of the iridoid and indole precursors of terpenoid indole
alkaloids, which are present in a single crude extract. The system
consists of a column of LiChrospher 60 RP select B 5 my, 250x4 mm
(Merck) with an eluent of 1 % formic
acid-acetonitrile-trichloroacetic acid (100:10:0.25, v:v:w) at a
flow of 1.2 ml/min. In the suspension cultures of Catharanthus
roseus secologanin and tryptophan were detected. In the cultures
of Tabernaemontana divarica loganin, tryptophan, and tryptamine
accumulated.
| Original language | English |
|---|---|
| Journal | Planta Medica |
| Volume | 62 |
| Pages (from-to) | 278-280 |
| ISSN | 0032-0943 |
| Publication status | Published - 1996 |