Analysis of several irdoid and indole precursors of terpenoid indole alkaloids with a single HPLC run.

Denise Dagnino, Jan Schripsema, Robert Verpoorte

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    Abstract

    An isocratic HPLC system is described which allows the separation of the iridoid and indole precursors of terpenoid indole alkaloids, which are present in a single crude extract. The system consists of a column of LiChrospher 60 RP select B 5 my, 250x4 mm (Merck) with an eluent of 1 % formic acid-acetonitrile-trichloroacetic acid (100:10:0.25, v:v:w) at a flow of 1.2 ml/min. In the suspension cultures of Catharanthus roseus secologanin and tryptophan were detected. In the cultures of Tabernaemontana divarica loganin, tryptophan, and tryptamine accumulated.
    Original languageEnglish
    JournalPlanta Medica
    Volume62
    Pages (from-to)278-280
    ISSN0032-0943
    Publication statusPublished - 1996

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