A rapid method was developed for partial characterization of the replicase-encoding open reading frame 1 (ORF 1) of porcine reproductive and respiratory syndrome virus (PRRSV). It comprised long RT-PCR amplification of 11.1 kb (94%) of ORF 1, followed by restriction fragment length polymorphism analysis. The method was used to compare ORF 1 sequences of two divergent European-type PRRSV strains. Our results indicated that the structural and replicase parts of these two strains had evolved at overall similar rates.
|Publication status||Published - 2000|
- replicase gene
- long RT-PCR
- porcine reproductive and respiratory syndrome virus
Nielsen, H. S., Storgaard, T., & Oleksiewicz, M. B. (2000). Analysis of ORF 1 in European porcine reproductive and respiratory syndrome virus by long RT-PCR and restriction fragment length polymorphism (RFLP) analysis. Veterinary Microbiology, 76(3), 221-228.