Analysis of ORF 1 in European porcine reproductive and respiratory syndrome virus by long RT-PCR and restriction fragment length polymorphism (RFLP) analysis

H. S. Nielsen, Torben Storgaard, M.B. Oleksiewicz

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    A rapid method was developed for partial characterization of the replicase-encoding open reading frame 1 (ORF 1) of porcine reproductive and respiratory syndrome virus (PRRSV). It comprised long RT-PCR amplification of 11.1 kb (94%) of ORF 1, followed by restriction fragment length polymorphism analysis. The method was used to compare ORF 1 sequences of two divergent European-type PRRSV strains. Our results indicated that the structural and replicase parts of these two strains had evolved at overall similar rates.
    Original languageEnglish
    JournalVeterinary Microbiology
    Volume76
    Issue number3
    Pages (from-to)221-228
    ISSN0378-1135
    Publication statusPublished - 2000

    Keywords

    • replicase gene
    • long RT-PCR
    • porcine reproductive and respiratory syndrome virus
    • RFLP

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