Abstract
The induction of the heat shock response in Lactococcus lactis
subsp. cremoris strain MG1363 was analysed at the RNA level using
a novel RNA isolation procedure to prevent degradation. Cloning of
the dnaJ and groEL homologous was carried out. Nothern blot
analysis showed a similar induction pattern for dnaK, dnaJ and
groELS after transfer from 30°C to 43°C when MG1363 was grown in
defined medium. The dnaK gene showed a 100-fold induction level 15
min after temperature shifting. Induction of the first two genes
in the dnaK operon, orf1 and grpW, resembled the pattern observed
for the above genes, although maximum induction was observed
earlier for orf1 and grpE. Novel transcript sizes were detected in
heat-shocked cells. The induction kinetics observed for ftsH
suggested a different regulation for this gene. Experimental
evidence for a prenounced transcriptional regulation being
involved in the heat shock response in L. lactis MG1363 is
presented. A gene located downstream of the dnaK operon in strain
MG1363, named orf4, was shown not to be regulated by heat shock.
| Original language | English |
|---|---|
| Journal | Microbiology |
| Volume | 142 |
| Issue number | 7 |
| Pages (from-to) | 1685-1691 |
| Publication status | Published - 1996 |