Analysis of four polycyclic aromatic hydrocarbons in plant-based food supplements-results of a collaborative study

Amelie Sina Wilde, Lene Duedahl-Olesen*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The determination of carcinogenic polycyclic aromatic hydrocarbons (PAHs) in food supplements is challenging, especially due to the presence of other e.g. heterogeneous PAH-like compounds in the matrix. A collaborative study with 12 participants was conducted in order to assess performance characteristics of a fast method intended to analyse the four regulated PAHs (PAH 4) benzo[b]fluoranthene [BbF], benz[a]anthracene [BaA], chrysene [CHR] and benzo[a]pyrene [BaP] in five different plant-based food supplements in the form of capsules, powder, and tablets. The principle of the method includes the extraction of PAHs with ethyl acetate: cyclohexane followed by a two-step SPE cleanup and final analysis by GC-MS or LC-FLD. The regulated maximum level for BaP is 10 µg/kg and, for the PAH 4, 50 µg/kg. Accordingly, the method was validated for the regulated PAH 4 analytically challenging concentration range from 2.5 µg/kg to 6.9 µg/kg. The performance criteria for the method set in European Regulation No 333/2007 for the overall repeatability, reproducibility (HorRat values below 2), and recovery (range 50-120%) were fulfilled. Based on the statistical evaluation of the results, it was concluded that the method is a suitable alternative to existing methods and should be studied for additional matrices.
Original languageEnglish
JournalFood Additives & Contaminants: Part A - Chemistry, Analysis, Control, Exposure & Risk Assessment
Volume39
Issue number8
Pages (from-to)1380-1389
Number of pages10
ISSN1944-0049
DOIs
Publication statusPublished - 2022

Keywords

  • PAH
  • Food supplements
  • SPE
  • Collaborative trial
  • GCMS
  • LC-FLD

Fingerprint

Dive into the research topics of 'Analysis of four polycyclic aromatic hydrocarbons in plant-based food supplements-results of a collaborative study'. Together they form a unique fingerprint.

Cite this