Analysis of dDNP NMR metabolic data from cancer cells

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Abstract

With the rise of the field of systems biology, metabolomic data have been integrated with the data for other -omic sciences, and these gigantic collections of correlated data have with the ever improving computing power, been data mined to locate biomarkers and motifs.[1] In this project the metabolic fingerprint of four prostate cancer cell lines, with different levels of aggression were analyzed. Metabolic data were obtained by incubating the cells with 13C6-d7 isotope labeled glucose, then quenching the metabolism, removing the cell debris and hyperpolarizing the metabolite extracts with dissolution Dynamic Nuclear Polarization (dDNP).
By integrating the peaks of the resulting NMR spectra, a collection of metabolic data was obtained without the need for identification of specific compounds. On this data, data mining was applied, with the aim to identify biomarkers of cancer and to classify the aggressiveness of the cancer. The illustrations below show examples of obtained NMR spectra for the different cell types (on the left) and Principal Components-Discriminant Function Analysis (PCDFA) results from the four prostate cancer cell types and a breast cancer cell line, in red, (on the right). The PC-DFA is clearly able to separate the cell types, with the most aggressive clustering together (blue and green). As dDNP MNR have been shown to be quantitative and reproducible,[2] it could be an important tool in the future for cancer diagnostics.
Original languageEnglish
Publication date2020
Number of pages1
Publication statusAccepted/In press - 2020
EventInternational Conference on Nuclear Hyperpolarization 2018 - Grand Harbour Hotel, Southampton , United Kingdom
Duration: 2 Sep 20185 Sep 2018

Conference

ConferenceInternational Conference on Nuclear Hyperpolarization 2018
LocationGrand Harbour Hotel
CountryUnited Kingdom
CitySouthampton
Period02/09/201805/09/2018

Cite this

Frahm, A. B., Lundsgaard, E., Matei, A., Jensen, P. R., Karlsson, M., Ardenkjær-Larsen, J. H., & Lerche, M. H. (Accepted/In press). Analysis of dDNP NMR metabolic data from cancer cells. Abstract from International Conference on Nuclear Hyperpolarization 2018, Southampton , United Kingdom.