Analysis of carbohydrate-carbohydrate interactions using sugar-functionalized silicon nanoparticles for cell imaging

Chian-Hui Lai, Julia Hütter, Chien-Wei Hsu, Hidenori Tanaka, Silvia Varela-Aramburu, Luisa De Cola, Peter H. Seeberger

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Protein-carbohydrate binding depends on multivalent ligand display that is even more important for low affinity carbohydrate-carbohydrate interactions. Detection and analysis of these low affinity multivalent binding events are technically challenging. We describe the synthesis of dual-fluorescent sugar-capped silicon nanoparticles that proved to be an attractive tool for the analysis of low affinity interactions. These ultra-small NPs with sizes of around 4 nm can be used for NMR quantification of coupled sugars. The silicon nanoparticles are employed to measure the interaction between the cancer-associated glycosphingolipids GM3 and Gg3 and the associated kD value by surface plasmon resonance experiments. Cell binding studies, to investigate the biological relevance of these carbohydrate-carbohydrate interactions, also benefit from these fluorescent sugar-capped nanoparticles.
Original languageEnglish
JournalNano Letters
ISSN1530-6984
DOIs
Publication statusAccepted/In press - 2015
Externally publishedYes

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